Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China.
Department of Chemistry, Shanxi Medical University, Taiyuan 030001, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 15;234:118251. doi: 10.1016/j.saa.2020.118251. Epub 2020 Mar 11.
Herein, nitrogen-doped carbon dots (N-CDs) emitting blue fluorescence were prepared using L-tartaric acid and triethylenetetramine through a simple and quick microwave-assisted method. The synthesized N-CDs displayed excitation-dependent fluorescence behavior, and their maximum excitation and emission wavelengths were 350 and 425 nm, respectively. The obtained N-CDs, which featured excellent fluorescence properties with a high fluorescence quantum yield of 31%, were applied to detect metronidazole (MNZ), which can effectively quench the fluorescence intensity of N-CDs due to the inner filter effect. This phenomenon was used as basis to develop a label-free fluorescent method for rapid MNZ determination, with the limit of detection of 0.22 μM and corresponding linear range of 0.5-22 μM. Hence, we had established a fluorescence method for MNZ detection and applied it to detect MNZ in real samples with satisfactory results. Finally, N-CDs with superior biocompatibility were applied for cell imaging and MNZ detection by the changes in fluorescence intensity.
本文采用 L-酒石酸和三乙烯四胺通过简单快速的微波辅助法制备了发蓝色荧光的氮掺杂碳点（N-CDs）。所合成的 N-CDs 表现出激发依赖性荧光行为，其最大激发和发射波长分别为 350nm 和 425nm。所得到的 N-CDs 具有出色的荧光性质，荧光量子产率高达 31%，可用于检测甲硝唑（MNZ），由于内滤效应，甲硝唑可有效猝灭 N-CDs 的荧光强度。基于此现象，建立了一种快速检测甲硝唑的无标记荧光方法，其检测限为 0.22μM，相应的线性范围为 0.5-22μM。因此，我们建立了一种用于检测甲硝唑的荧光方法，并将其应用于实际样品中检测甲硝唑，结果令人满意。最后，利用 N-CDs 的优异生物相容性，通过荧光强度的变化进行细胞成像和甲硝唑检测。
