College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, 310015, China.
Hangzhou Botanical Garden, Hangzhou, 310007, China.
Sci Rep. 2020 Mar 19;10(1):4996. doi: 10.1038/s41598-020-61951-x.
Corydalis Rhizoma is the tuber of Corydalis yanhusuo W. T. Wang, which has been long used in traditional Chinese medicine. Herein, the quality of C. yanhusuo samples collected from 23 regions of three provinces in China is evaluated through high-performance liquid chromatography fingerprinting coupled with similarity, hierarchical clustering, and principal component analyses. Sample similarities are evaluated according to the State Food and Drug Administration requirements by selection of 18 characteristic chromatographic fingerprint peaks and are found to vary between 0.455 and 0.999. Moreover, common patterns of a typical local variety of C. yanhusuo sourced in the Panan County are established. The obtained results show that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of C. yanhusuo and related products.
延胡索是罂粟科紫堇属植物延胡索的干燥块茎，在中医药中已有悠久的应用历史。本研究采用高效液相色谱指纹图谱结合相似度评价、层次聚类分析和主成分分析方法，对来自中国三省 23 个地区的延胡索样品进行了质量评价。根据国家食品药品监督管理局的要求，选择 18 个特征色谱指纹峰进行评价，样品相似度在 0.455 到 0.999 之间。此外，还建立了磐安县当地特有的延胡索典型品种的共有模式。研究结果表明，定量分析与指纹图谱分析相结合可用于质量控制，为延胡索及其相关产品的质量评价提供了全面的策略。
