State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715, China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715, China; College of Life Sciences, Chongqing Normal University, Chongqing 400047, China.
J Invertebr Pathol. 2020 May;172:107350. doi: 10.1016/j.jip.2020.107350. Epub 2020 Mar 17.
Nosema bombycis, the first identified microsporidium, causes heavy losses to the sericulture industry in China. During infection, microsporidia discharge a long and hollow polar tube, which delivers the sporoplasm into host cells. Polar tube protein 1 was the major component on the polar tube. Previously, we expressed the polar tube protein 1 from Nosema bombycis (NbPTP1) intercellularly in Drosophila S2 cells. Here, the microsporidian protein was expressed in Lepidopteran Sf9 cells. During heterologous expression, NbPTP1 protein was secreted and glycosylated. Microsporidian proliferation decreased in NbPTP1-expressing Sf9 cells. This confirms that NbPTP1 protein can interact with the host cell membrane receptor protein to facilitate microsporidian invasion.
微孢子虫 Nosema bombycis 是最早被鉴定的微孢子虫，它会给中国的养蚕业造成严重损失。在感染过程中，微孢子虫会排出一条长而中空的极丝，将孢子质输送到宿主细胞中。极丝蛋白 1 是极丝的主要成分。此前，我们在果蝇 S2 细胞中胞内表达了来自 N. bombycis 的极丝蛋白 1（NbPTP1）。在这里，该微孢子虫蛋白在鳞翅目 Sf9 细胞中表达。在异源表达过程中，NbPTP1 蛋白被分泌并发生糖基化。表达 NbPTP1 的 Sf9 细胞中的微孢子虫增殖减少。这证实了 NbPTP1 蛋白可以与宿主细胞膜受体蛋白相互作用，从而促进微孢子虫的入侵。
