Instituto de Microbiología y Zoología Agrícola (IMYZA), Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Hurlingham, Buenos Aires, Argentina.
Instituto de Microbiología y Zoología Agrícola (IMYZA), Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.
J Invertebr Pathol. 2020 May;172:107354. doi: 10.1016/j.jip.2020.107354. Epub 2020 Mar 17.
Bacillus thuringiensis toxins of the Cry1I class have dual specificity for insects in the orders Coleoptera and Lepidoptera. We assessed the toxicity of a Cry1Ia protein from an Argentinian B. thuringiensis strain against agricultural pests in the families Tenebrionidae, Curculionidae, Noctuidae and Tortricidae. Three recombinant protein variants were produced that differed in length and fusion tag position to rule out artifactual results. The protein was toxic to Cydia pomonella and Rachiplusia nu. In contrast, Alphitobius diaperinus, Anthonomus grandis and Spodoptera frugiperda were not susceptible. The results are discussed with respect to previous studies and the prospective use of Cry1Ia in strategies to control major cotton pests in the region.
苏云金芽孢杆菌 Cry1I 类毒素对鞘翅目和鳞翅目昆虫具有双重特异性。我们评估了来自阿根廷苏云金芽孢杆菌菌株的 Cry1Ia 蛋白对夜蛾科、象甲科、夜蛾科和卷叶蛾科农业害虫的毒性。产生了三种重组蛋白变体，它们在长度和融合标签位置上有所不同，以排除人为结果。该蛋白对苹果实蝇和小菜蛾具有毒性。相比之下，黄粉甲、大猿叶甲和玉米穗虫则不易受感染。结果与以前的研究以及 Cry1Ia 在该地区控制主要棉花害虫策略中的潜在用途进行了讨论。
