Unit of Orthodontics, Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via San Vitale 59, 40125, Bologna, Italy.
Preclinical and Surgical Studies Laboratory, Rizzoli RIT Department, IRCCS - Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136, Bologna, Italy.
Arch Biochem Biophys. 2020 May 30;685:108333. doi: 10.1016/j.abb.2020.108333. Epub 2020 Mar 16.
This study summarizes the available evidence from systematic reviews on the in vitro effects of photobiomodulation on the proliferation and differentiation of human bone and stromal cells by appraising their methodological quality. Improvements for future studies are also highlighted, with particular emphasis on in vitro protocols and cell-related characteristics. Six reviews using explicit eligibility criteria and methods selected in order to minimize bias were included. There was no compelling evidence on the cellular mechanisms of action or treatment parameters of photobiomodulation; compliance with quality assessment was poor. A rigorous description of laser parameters (wavelength, power, beam spot size, power density, energy density, repetition rate, pulse duration or duty cycle, exposure duration, frequency of treatments, and total radiant energy), exposure conditions (methods to ensure a uniform irradiation and to avoid cross-irradiation, laser-cell culture surface distance, lid presence during irradiation) and cell-related characteristics (cell type or line, isolation and culture conditions, donor-related factors where applicable, tissue source, cell phenotype, cell density, number of cell passages in culture) should be included among eligibility criteria for study inclusion. These methodological improvements will maximize the contribution of in vitro studies on the effects of photobiomodulation on human bone and stromal cells to evidence-based translational research.
本研究通过评估系统评价中关于光生物调节对体外人骨和基质细胞增殖和分化的影响的现有证据的方法学质量，总结了这些证据。还强调了未来研究的改进措施，特别强调了体外方案和细胞相关特征。纳入了 6 项使用明确纳入标准和方法的综述，以最大程度地减少偏倚。关于光生物调节的作用机制或治疗参数，没有确凿的证据；对质量评估的遵守情况较差。应在纳入研究的标准中包括对激光参数（波长、功率、光束光斑尺寸、功率密度、能量密度、重复率、脉冲持续时间或占空比、曝光持续时间、治疗频率和总辐射能）、暴露条件（确保均匀照射和避免交叉照射的方法、激光-细胞培养表面距离、照射时盖子的存在）和细胞相关特征（细胞类型或系、分离和培养条件、适用时供体相关因素、组织来源、细胞表型、细胞密度、培养中细胞传代的次数）进行严格描述。这些方法学改进将最大限度地提高体外研究对光生物调节对人骨和基质细胞影响的贡献，为基于证据的转化研究提供依据。
