College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Department of Food Science and Technology, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt.
Int J Biol Macromol. 2020 Jul 1;154:706-713. doi: 10.1016/j.ijbiomac.2020.03.146. Epub 2020 Mar 17.
Mung bean seeds were germinated at 25 °C for 12, 24, 36, 48, 60 and 72 h. Changes in structural and physicochemical properties of mung bean starch during germination were investigated. Microscopy analysis showed germination did not change the shape of starch granules, but granules isolated from germinated mung bean starch exhibited dents surface structure compared with smooth surface of native starch. The molecular weight of starch isolated from germinated mung bean was found to be smaller than that of native ones. Although germinated mung bean showed greater crystallinity than that of native sample, the germination did not change the crystalline structure type of starch (C-type). Moreover, solubility and swelling power of starch varied depending on germination duration and testing temperature. Peak viscosity and trough viscosity slightly decreased as germination prolonged to 72 h. Furthermore, starch isolated from mung bean germinated for 12 h showed greater enthalpy (ΔH) than that of starch isolated from native sample; while, ΔH decrease as germination time prolonged from 36 to 72 h. The obtained results may help in understanding the influence of germination on functional properties of mung bean starch and choosing appropriate applications to promote utilization of mung bean starch in food industry.
绿豆种子在 25°C 下发芽 12、24、36、48、60 和 72 小时。研究了发芽过程中绿豆淀粉的结构和物理化学性质的变化。显微镜分析表明,发芽并没有改变淀粉颗粒的形状,但与天然淀粉光滑的表面相比,从发芽绿豆淀粉中分离出的颗粒表面呈现出凹痕结构。从发芽绿豆中分离出的淀粉的分子量比天然淀粉的分子量小。尽管发芽绿豆的结晶度比天然样品高,但发芽并没有改变淀粉的结晶结构类型(C 型)。此外,淀粉的溶解度和膨胀力取决于发芽时间和测试温度。随着发芽时间延长至 72 小时,峰值粘度和低谷粘度略有下降。此外,发芽 12 小时的绿豆淀粉分离物的焓(ΔH)高于天然淀粉分离物的焓;而随着发芽时间从 36 小时延长至 72 小时,ΔH 下降。这些结果可能有助于理解发芽对绿豆淀粉功能特性的影响,并选择合适的应用来促进绿豆淀粉在食品工业中的应用。