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红茶和基质金属蛋白酶抑制剂对牙本质侵蚀的原位影响。

Effect of black tea and matrix metalloproteinase inhibitors on eroded dentin in situ.

机构信息

Department of Restorative Dentistry, Istanbul University, Istanbul, Turkey.

出版信息

Microsc Res Tech. 2020 Jul;83(7):834-842. doi: 10.1002/jemt.23475. Epub 2020 Mar 20.

Abstract

Purpose of this in situ study was to evaluate the surface properties of eroded dentin specimens activated with three different matrix metalloproteinase (MMP) inhibitors (chlorhexidine [CHX], fluoride, green tea), black tea, and water. One hundred eighty dentin samples were prepared from extracted third molars and then samples divided into six groups. Ten volunteers were carried three specimens of each group, on acrylic palatal appliances, which were fabricated exactly for them (n = 3). Erosive cycles were done by immersing appliances in cup containing Cola and was followed by rinsing with test solutions. Microhardness values were measured. Surface properties were investigated by atomic force microscopy (AFM). Lowest change in microhardness was shown in fluoride group whereas negative control group (water) had the highest change. There were no statistically significant differences among surface roughness changes (p > .05). The least change in microhardness was seen in the fluoride group (13.05 ± 8.07), while the control group showed the highest change (33.80 ± 12.42) and was statistically significant when compared to other groups (p < .05). Besides lowest depth, values were shown in fluoride group as well. AFM evaluations showed macromolecular deposits on surfaces of fluoride, CHX, and black tea groups. No superior results were detected in CHX + fluoride group and black tea showed similar surface characteristics as green tea. Mouthrinses containing not only green tea but also black tea could be beneficial for patients with exposed dentin surfaces. Catechines and theaflavins in teas could be useful for improving surface quality.

摘要

本原位研究的目的是评估用三种不同基质金属蛋白酶 (MMP) 抑制剂(洗必泰 [CHX]、氟化物、绿茶)、红茶和水激活后的被侵蚀牙本质标本的表面特性。从提取的第三磨牙中制备了 180 个牙本质样本,然后将样本分为六组。10 名志愿者每人携带三组每组的样本,放在为他们精确制作的丙烯酸腭托上(n = 3)。通过将器械浸入含有可乐的杯中进行侵蚀循环,然后用测试溶液冲洗。测量显微硬度值。通过原子力显微镜 (AFM) 研究表面特性。氟化物组显示出最低的显微硬度变化,而阴性对照组(水)的变化最大。表面粗糙度变化之间没有统计学上的显著差异(p>0.05)。氟化物组的显微硬度变化最小(13.05±8.07),而对照组显示出最大的变化(33.80±12.42),与其他组相比具有统计学意义(p<0.05)。除了最小的深度值外,氟化物组也显示出最小的硬度值。AFM 评估显示氟化物、CHX 和红茶组表面有大分子沉积物。CHX+氟化物组未检测到更优的结果,红茶显示出与绿茶相似的表面特性。含有不仅绿茶而且还有红茶的漱口水可能对暴露牙本质表面的患者有益。茶中的儿茶素和茶黄素可能有助于改善表面质量。

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