Lazarchick J, Jones T J, Russell R J, Hall S A
Department of Pathology, Medical University of South Carolina, Charleston 29425.
Diagn Clin Immunol. 1988;5(6):377-80.
The immunoreactivity of five sera containing either iso-, auto- or allo-antiplatelet antibodies to frozen-thawed human lymphocyte antigen (HLA)-typed pooled platelets was assessed to determine the practicality of using such a platelet preparation in an immunofluorescent platelet antibody system. The platelet reagent was analyzed over an 8-week period after initial freezing and storage. Mixtures of platelets with each serum or dilutions of it were initially incubated for 1 hour, then extensively washed and incubated with fluorescein-conjugated goat F(ab')2 antihuman IgG, A, M. After repeat washing, each mixture was then analyzed using a flow cytometer to determine the relative amount of fluorescent antibody bound. Not only was each of the five sera consistently positive for the presence of an anti-platelet antibody, but the extent of immunoreactivity for each remained relatively stable over the 8-week period of analysis. The results suggest that frozen pooled HLA-typed platelets should be an invaluable reagent for screening serum samples for the presence of antiplatelet antibodies in patients who are thrombocytopenic or who are refractory to platelet transfusion therapy.
评估了五份含有同种、自身或同种异体抗血小板抗体的血清与冻融的人类淋巴细胞抗原（HLA）分型混合血小板的免疫反应性，以确定在免疫荧光血小板抗体系统中使用这种血小板制剂的实用性。在初次冷冻和储存后的8周内对血小板试剂进行了分析。血小板与每种血清或其稀释液的混合物最初孵育1小时，然后充分洗涤，再与荧光素偶联的山羊F(ab')2抗人IgG、A、M孵育。再次洗涤后，使用流式细胞仪分析每种混合物，以确定结合的荧光抗体的相对量。这五份血清中的每一份不仅始终显示存在抗血小板抗体呈阳性，而且在8周的分析期内，每份血清的免疫反应程度保持相对稳定。结果表明，冷冻的HLA分型混合血小板对于筛查血小板减少症患者或对血小板输血治疗无效患者的血清样本中是否存在抗血小板抗体应是一种非常有价值的试剂。
