Department of Oral Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, People's Republic of China.
Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, 250001, People's Republic of China.
Sci Rep. 2020 Mar 20;10(1):5172. doi: 10.1038/s41598-020-62107-7.
Delayed resorption of crown-covered bone is a critical cause of delayed tooth eruption. Traditional herbal medicines may be good auxiliary treatments to promote the resorption of crown-covered bone. This study was carried out to analyse the effect of isorhamnetin 3-O-neohesperidoside on receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis in vitro and resorption of the crown-covered bone of the lower first molars in mice in vivo. Isorhamnetin 3-O-neohesperidoside promoted osteoclastogenesis and the bone resorption of mouse bone marrow macrophages (BMMs) and upregulated mRNA expression of the osteoclast-specific genes cathepsin K (CTSK), vacuolar-type H + -ATPase d2(V-ATPase d2), tartrate resistant acid phosphatase (TRAP) and nuclear factor of activated T-cells cytoplasmic 1 (NFATc1). NFATc1, p38 and AKT signalling was obviously activated by isorhamnetin 3-O-neohesperidoside in osteoclastogenesis. Isorhamnetin 3-O-neohesperidoside aggravated resorption of crown-covered bone in vivo. In brief, isorhamnetin 3-O-neohesperidoside might be a candidate adjuvant therapy for delayed intraosseous eruption.
牙冠骨吸收延迟是牙齿迟萌的关键原因。传统草药可能是促进牙冠骨吸收的良好辅助治疗方法。本研究旨在分析山柰酚 3-O-新橙皮糖苷对核因子κB 受体激活剂配体（RANKL）诱导的体外破骨细胞发生和体内小鼠下第一磨牙牙冠骨吸收的影响。山柰酚 3-O-新橙皮糖苷促进了破骨细胞形成和小鼠骨髓巨噬细胞（BMMs）的骨吸收，并上调了破骨细胞特异性基因组织蛋白酶 K（CTSK）、液泡型 H+ -ATP 酶 d2（V-ATPase d2）、抗酒石酸酸性磷酸酶（TRAP）和激活 T 细胞核因子 1（NFATc1）的 mRNA 表达。在破骨细胞发生过程中，山柰酚 3-O-新橙皮糖苷明显激活了 NFATc1、p38 和 AKT 信号通路。山柰酚 3-O-新橙皮糖苷加重了体内牙冠骨的吸收。总之，山柰酚 3-O-新橙皮糖苷可能是一种治疗牙内萌出延迟的候选辅助治疗方法。
