肾移植的分子评估：我们是否更接近日常常规？
Molecular Assessment of Kidney Allografts: Are We Closer to a Daily Routine?
机构信息
Department of Nephrology, Transplant Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
出版信息
Physiol Res. 2020 Apr 30;69(2):215-226. doi: 10.33549/physiolres.934278. Epub 2020 Mar 23.
Abstract
Kidney allograft pathology assessment has been traditionally based on clinical and histological criteria. Despite improvements in Banff histological classification, the diagnostics in particular cases is problematic reflecting a complex pathogenesis of graft injuries. With the advent of molecular techniques, polymerase-chain reaction, oligo- and microarray technologies allowed to study molecular phenotypes of graft injuries, especially acute and chronic rejections. Moreover, development of the molecular microscope diagnostic system (MMDx) to assess kidney graft biopsies, represents the first clinical application of a microarray-based method in transplantation. Whether MMDx may replace conventional pathology is the subject of ongoing research, however this platform is particularly useful in complex histological findings and may help clinicians to guide the therapy.
摘要
肾移植病理学评估传统上基于临床和组织学标准。尽管班夫组织学分类有所改进，但在某些特定情况下的诊断仍然存在问题，反映出移植物损伤的复杂发病机制。随着分子技术的出现，聚合酶链反应、寡核苷酸和微阵列技术使得研究移植物损伤的分子表型成为可能，特别是急性和慢性排斥反应。此外，开发用于评估肾移植活检的分子显微镜诊断系统 (MMDx) 代表了基于微阵列方法在移植中的首次临床应用。MMDx 是否可以替代传统病理学是正在进行的研究的主题，然而，该平台在复杂的组织学发现中特别有用，并可能有助于临床医生指导治疗。
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