School of Environment, Guangdong Engineering Research Center of Water Treatment Processes and Materials, And Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.
School of Environment, Guangdong Engineering Research Center of Water Treatment Processes and Materials, And Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.
Chemosphere. 2020 Aug;252:126378. doi: 10.1016/j.chemosphere.2020.126378. Epub 2020 Mar 11.
Biofilm based systems and the hybrid between activated sludge and biofilms have been popularly applied for wastewater treatment. Unlike the suspended biomass, the biofilm concentration and kinetics on the media cannot be easily measured. In this study, a novel and easy-to-use approach has been developed based on pulse-flow respirometer to characterize the biofilm stoichiometry and kinetics in situ. With the new designed breathing reactor, the mutual interference between the magnetic stirring and biofilm media that happened in the conventional breathing reactor was solved. Moreover, Microsoft Excel based programs had been developed to fit the oxygen uptake rate curves with dynamic nonlinear regression. With this new approach, the yield coefficient, maximum oxidation capacity, and half-saturation constant of substrate for the heterotrophic biofilms in a fix bed reactor were determined to be 0.46 g-VSS/g-COD, 67.0 mg-COD/(h·L-media), and 4.4 mg-COD/L, respectively. Those parameters for biofilm ammonia oxidizers from a moving bed biofilm reactor were determined to be 0.17 g-VSS/g-N, 18.6 mg-N/(h·L-media), and 1.2 mg-N/L, respectively, and they were 0.11 g-VSS/g-N, 20.9 mg-N/(h·L-media), and 0.98 mg-N/L for nitrite oxidizers in the same biofilms. This study also found that the maximum specific substrate utilization rate for detached biofilms increased by 3.2 times, indicating that maintaining biofilm integrity was very important in the kinetic tests. Using this approach, the biofilm kinetics on the media can be regularly measured for treatment optimization.
基于生物膜的系统和活性污泥与生物膜之间的混合体已广泛应用于废水处理。与悬浮生物量不同，生物膜在介质上的浓度和动力学不易测量。在本研究中，基于脉冲流呼吸计开发了一种新颖且易于使用的方法，用于原位表征生物膜化学计量和动力学。使用新设计的呼吸反应器，解决了传统呼吸反应器中磁搅拌和生物膜介质之间的相互干扰问题。此外，还开发了基于 Microsoft Excel 的程序，以动态非线性回归拟合耗氧率曲线。通过这种新方法，确定固定床反应器中异养生物膜的产率系数、最大氧化能力和基质半饱和常数分别为 0.46 g-VSS/g-COD、67.0 mg-COD/（h·L-介质）和 4.4 mg-COD/L。移动床生物膜反应器中生物膜氨氧化菌的这些参数分别为 0.17 g-VSS/g-N、18.6 mg-N/（h·L-介质）和 1.2 mg-N/L，而同一生物膜中的亚硝酸盐氧化菌分别为 0.11 g-VSS/g-N、20.9 mg-N/（h·L-介质）和 0.98 mg-N/L。本研究还发现，游离生物膜的最大比基质利用速率增加了 3.2 倍，这表明保持生物膜完整性在动力学测试中非常重要。使用这种方法，可以定期测量介质上的生物膜动力学，以优化处理。
