Grupo de investigación Histopatología, Facultad de Medicina, Universidad de Cartagena, Carrera 50 # 24-120, código postal 130014 Cartagena, Colombia.
Grupo de investigación Histopatología, Facultad de Medicina, Universidad de Cartagena, Carrera 50 # 24-120, código postal 130014 Cartagena, Colombia.
Rev Esp Patol. 2020 Apr-Jun;53(2):79-87. doi: 10.1016/j.patol.2019.06.005. Epub 2019 Aug 28.
The association between Helicobacter-pylori-induced inflammation and gastric adenocarcinoma is well documented and it has been suggested that the pro-mitotic and apoptotic effect of Cyclooxygenase-2 and Osteopontin on the epithelial cells of the gastric mucosa may have a role in carcinogenesis of the gastric mucosa. The aim of this study was to determine the expression of Cyclooxygenase-2 and Osteopontin in normal gastric mucosa, mucosa with gastritis and gastric mucosa with intestinal metaplasia, in relation to Helicobacter-pylori infection and grade of inflammation. Immunohistochemistry was performed on 108 gastric biopsies in order to detect Cyclooxygenase-2 and Osteopontin expression. The intensity and percentage of staining were evaluated using the H-Score, and its association with grade of inflammation, Helicobacter pylori infection and intestinal metaplasia was determined. Expression of Cyclooxygenase-2 and Osteopontin was higher in gastric biopsies (values shown respectively) with Helicobacter-pylori infection (179.9/142.3), intestinal metaplasia (208.8/179.3) or higher grades of inflammation (190/135.7) in comparison to normal gastric mucosa (100.7/80) or mild grade of inflammation (128.4/128.4), (p<0.05).There is an overexpression of Cyclooxygenase-2 and Osteopontin in gastric mucosa with H. pylori infection, intestinal metaplasia and high grades of inflammation, suggesting a constant up-regulation of protein expression in response to the inflammatory process generated by a Helicobacter-pylori infection, leading to the development of intestinal metaplasia.
幽门螺杆菌引起的炎症与胃腺癌之间的关联已有充分的文献记载，并且有人提出环氧化酶-2 和骨桥蛋白对胃黏膜上皮细胞的促有丝分裂和凋亡作用可能在胃黏膜癌变中起作用。本研究旨在确定环氧化酶-2 和骨桥蛋白在正常胃黏膜、胃炎黏膜和肠上皮化生胃黏膜中的表达与幽门螺杆菌感染和炎症程度的关系。为了检测环氧化酶-2 和骨桥蛋白的表达，对 108 例胃活检组织进行了免疫组织化学染色。使用 H 评分评估染色强度和百分比，并确定其与炎症程度、幽门螺杆菌感染和肠上皮化生的相关性。在有幽门螺杆菌感染（分别为 179.9/142.3）、肠上皮化生（208.8/179.3）或更高炎症程度（190/135.7）的胃活检组织中，环氧化酶-2 和骨桥蛋白的表达高于正常胃黏膜（分别为 100.7/80）或轻度炎症程度（128.4/128.4）（p<0.05）。在有幽门螺杆菌感染、肠上皮化生和高炎症程度的胃黏膜中，环氧化酶-2 和骨桥蛋白表达过度，提示蛋白表达持续上调以应对幽门螺杆菌感染引起的炎症反应，从而导致肠上皮化生的发展。
