Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, 88040-900, Brazil.
Department of Food, Technical College of Campinas, University of Campinas, Campinas, São Paulo, 13083-862, Brazil.
Appl Microbiol Biotechnol. 2020 May;104(10):4235-4246. doi: 10.1007/s00253-020-10526-x. Epub 2020 Mar 21.
The population growth is causing an increase in the generation of effluents (mainly organic fraction of municipal solid waste (OFMSW) and agro-industrial waste), which is an old problem in agro-industrial countries such as Brazil. Contrastingly, it is possible to add value to these residual biomasses (residues) through the application of new technologies for the production of bioenergy. Anaerobic digestion (AD) of sewage sludge is being applied in many effluent treatment plants for the sustainable and economically viable production of biogas. However, the biogas produced from AD (sludge) or co-digestion (sludge with other residues) presents a concentration of methane between 60 and 70% on average, which is relatively low. This review is aimed at analyzing studies involving (i) production of lipases by solid-state fermentation (SSF) by different microorganisms for the application in enzymatic pretreatments prior to the anaerobic treatment of effluents; (ii) pretreatment followed by AD of various residues, with an emphasis on OFMSW and sewage sludge; and (iii) more recent studies on anaerobic co-digestion (AcoD) and hybrid technologies (pretreatment + AD or AcoD). There are many studies in the literature that demonstrate the enzymatic pretreatment or AcoD applied to the optimization of methane production. Nevertheless, few studies report the combination of these two technologies, which can improve the process and reduce or eliminate the costs of biogas purification, which are major challenges for the viability of this route of bioenergy production. KEY POINTS: • Municipal and agro-industrial wastes have potential as medium for lipase production. • Enzymatic pretreatment and anaerobic co-digestion are low cost for high-methane production. Graphical abstract Interactions among various factors optimization methane production from enzymatic pretreatment and AcoD.
人口增长导致污水（主要为城市固体废物的有机部分（OFMSW）和农业工业废物）的产生量增加，这在巴西等农业工业国家是一个老问题。相比之下，可以通过应用生产生物能源的新技术为这些残余生物质（残留物）增值。许多污水厂正在应用厌氧消化（AD）来可持续且经济可行地生产沼气。然而，由 AD（污泥）或共消化（污泥与其他残留物）产生的沼气的甲烷浓度平均在 60%到 70%之间，相对较低。本文旨在分析涉及（i）不同微生物通过固态发酵（SSF）生产脂肪酶，以应用于污水厌氧处理前的酶预处理；（ii）各种残留物的预处理后 AD，重点是 OFMSW 和污水污泥；（iii）最近关于厌氧共消化（AcoD）和混合技术（预处理+AD 或 AcoD）的研究。文献中有许多研究表明，酶预处理或 AcoD 可用于优化甲烷产量。然而，很少有研究报告这两种技术的结合，这可以改进该工艺并降低或消除沼气净化的成本，这是生物能源生产这一途径的可行性的主要挑战。要点：• 城市和农业工业废物具有作为脂肪酶生产介质的潜力。• 酶预处理和厌氧共消化对于高产甲烷的低成本。 从酶预处理和 AcoD 优化甲烷生产的各种因素之间的相互作用。
