Çanakkale Onsekiz Mart University, Faculty of Engineering, Bioengineering Department, Çanakkale, Turkey.
Talanta. 2020 Jun 1;213:120779. doi: 10.1016/j.talanta.2020.120779. Epub 2020 Jan 23.
Troponin T, which is a biomarker of cardiovascular disease, is vital for acute myocardial infarction (AMI). AMI is the most well-known cause of death in the world. In the present work, we designed a novel immunosensor using disposable, cheap, and very conductive graphite paper electrodes (GP). The proposed immunosensor was modified with hydrochloric acid (HCl) for immobilization of anti-TnT antibody. After carboxyl group formation on the surface, the GP electrodes were incubated with EDC/NHS pair as a cross-linker. Electrochemical impedance spectroscopy (EIS), single frequency impedance (SFI), cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used for electrochemical characterization of the label-free immunosensor. Furthermore, the fabrication process of the proposed immunosensor was examined by using scanning electron microscopy (SEM). The proposed electrochemical immunosensor had a wide detection range (0.5 fg - 1000 fg/mL), and low limit of detection (LOD) and low limit of quantification (LOQ) at 1.28 fg/mL and 4.29 fg/mL, respectively. The designed immunosensor exhibited very good sensitivity, reproducibility, repeatability, reusability and long storage life. Additionally, the immunosensor was successfully applied to detection of TnT in human serum.
肌钙蛋白 T 是心血管疾病的生物标志物，对急性心肌梗死（AMI）至关重要。AMI 是世界上最著名的死亡原因。在本工作中，我们设计了一种使用一次性、廉价且非常导电的石墨纸电极（GP）的新型免疫传感器。该免疫传感器经过盐酸（HCl）修饰，用于固定抗 TnT 抗体。在表面形成羧基后，将 GP 电极用 EDC/NHS 对作为交联剂孵育。电化学阻抗谱（EIS）、单频阻抗（SFI）、循环伏安法（CV）和方波伏安法（SWV）技术用于无标记免疫传感器的电化学表征。此外，还通过扫描电子显微镜（SEM）检查了所提出的免疫传感器的制造过程。所提出的电化学免疫传感器具有较宽的检测范围（0.5 fg-1000 fg/mL），检测限（LOD）和定量下限（LOQ）分别低至 1.28 fg/mL 和 4.29 fg/mL。设计的免疫传感器表现出非常好的灵敏度、重现性、重复性、可重复性和长储存寿命。此外，该免疫传感器成功地应用于检测人血清中的 TnT。
