Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Anal Biochem. 2020 May 15;597:113693. doi: 10.1016/j.ab.2020.113693. Epub 2020 Mar 19.
The spherical sepharose CL-6B beads were activated by epichlorohydrin in different epoxy contents (80, 120 and 160 μmol/mL) and, l-histidine and imidazole as pseudo-affinity ligands were covalently immobilized to them. Some linkers with different length, (1,2-ethanediol diglycidyl ether and 1,4-butanediol diglycidyl ether) were synthesized for activation of sepharose and the activated sepharose beads modified with imidazole and the performance of these adsorbents in the purification of immunoglobulin G from bovine milk were evaluated. Among the l-histidine bearing adsorbents, higher adsorption of IgG (0.28 mg/mL) was obtained by adsorbent with the lower concentration of l-histidine. The highest amount of IgG adsorption (0.53 mg/mL) was obtained by imidazole bearing adsorbent with the highest amount of imidazole and Among the adsorbents with synthesized linkers, the adsorbent with 1,2-ethanediol diglycidyl ether showed better performance and was able to purify 0.25 mg/mL IgG with high purity. The synthesized pseudo-affinity adsorbents represented the abbility to purify immunoglobulin G in one-step process with high purity and efficiency.
球形琼脂糖 CL-6B 珠粒用不同环氧基含量（80、120 和 160 μmol/mL）的表氯醇进行活化，然后将 l-组氨酸和咪唑作为伪亲和配体共价固定在其上。合成了一些具有不同长度的连接子（1,2-乙二胺二缩水甘油醚和 1,4-丁二醇二缩水甘油醚）用于琼脂糖的活化，并用咪唑对活化的琼脂糖珠粒进行修饰，并评估了这些吸附剂在从牛初乳中纯化免疫球蛋白 G 方面的性能。在携带 l-组氨酸的吸附剂中，l-组氨酸浓度较低的吸附剂对 IgG 的吸附率较高（0.28 mg/mL）。携带咪唑的吸附剂对 IgG 的吸附量最高（0.53 mg/mL），其咪唑含量也最高。在合成的连接子的吸附剂中，1,2-乙二胺二缩水甘油醚的吸附剂表现出更好的性能，能够以高纯度纯化 0.25 mg/mL IgG。合成的伪亲和吸附剂具有在一步法中以高纯度和高效率纯化免疫球蛋白 G 的能力。
