在扇贝中鉴定 Kdm 基因的表达谱表明组蛋白去甲基化在早期发育和配子发生的调控中起作用。
Expression profiling of the Kdm genes in scallop Patinopecten yessoensis suggests involvement of histone demethylation in regulation of early development and gametogenesis.
机构信息
MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China.
MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 2020 Jun;243-244:110434. doi: 10.1016/j.cbpb.2020.110434. Epub 2020 Mar 19.
Histone demethylation modification is an important means of gene expression regulation and is widely involved in biological processes such as animal reproduction and development. Histone lysine demethylases (Kdm) plays an important role in the demethylation of histones. To understand the role of histone demethylation in scallops, we identified the Kdm gene family of the Yesso scallop Patinopecten yessoensis, and analyzed its expression during the gonad and early development. The results showed that the P. yessoensis has a complete Kdm family including seventeen members that belong to sixteen subfamilies (Hif1an, Hspbap1, Jarid2, Jmjd4, Jmjd6, Jmjd7, Jmjd8, Kdm1, Kdm2, Kdm3, Kdm4, Kdm5, Kdm6, Kdm7, Kdm8 and Kdm9). The Kdm genes showed five different expression patterns in the early development of scallop, with some of them (e.g. Jmjd7, Jmjd8 and Kdm8) being highly expressed in only one or two stage and the others (e.g. Kdm1A, Kdm9, Jmjd4 and Jmjd6) in several consecutive stages. During gonadal development, the Kdm genes also display various expression patterns. Some genes (e.g. Kdm2, Kdm4 and Jmjd7) display preferential expression in the testis, and the others have no obvious sex bias but show stage preference (resting, proliferative, growing or maturation stage). These results suggest that various histone demethylation modifications (e.g. H3K4, H3K9 and H3K27) may participate in the regulation of gametogenesis and early development of Yesso scallop. It will facilitate a better understanding of the epigenetic contributions to mollusk development.
组蛋白去甲基化修饰是基因表达调控的重要手段，广泛参与动物繁殖和发育等生物学过程。组蛋白赖氨酸去甲基酶（Kdm）在组蛋白去甲基化中发挥重要作用。为了了解组蛋白去甲基化在扇贝中的作用，我们鉴定了虾夷扇贝（Patinopecten yessoensis）的 Kdm 基因家族，并分析了其在性腺和早期发育过程中的表达。结果表明，虾夷扇贝具有完整的 Kdm 家族，包括 17 个成员，属于 16 个亚家族（Hif1an、Hspbap1、Jarid2、Jmjd4、Jmjd6、Jmjd7、Jmjd8、Kdm1、Kdm2、Kdm3、Kdm4、Kdm5、Kdm6、Kdm7、Kdm8 和 Kdm9）。Kdm 基因在扇贝早期发育过程中表现出五种不同的表达模式，其中一些基因（如 Jmjd7、Jmjd8 和 Kdm8）仅在一个或两个阶段高度表达，而另一些基因（如 Kdm1A、Kdm9、Jmjd4 和 Jmjd6）则在几个连续阶段表达。在性腺发育过程中，Kdm 基因也表现出不同的表达模式。一些基因（如 Kdm2、Kdm4 和 Jmjd7）在精巢中表现出优先表达，而其他基因没有明显的性别偏好，但表现出阶段偏好（休眠期、增殖期、生长期或成熟期）。这些结果表明，各种组蛋白去甲基化修饰（如 H3K4、H3K9 和 H3K27）可能参与了虾夷扇贝配子发生和早期发育的调控。这将有助于更好地理解表观遗传对软体动物发育的贡献。
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