Van Noten Noémie, Van Liefferinge Elout, Degroote Jeroen, De Smet Stefaan, Desmet Tom, Michiels Joris
Department of Animal Sciences and Aquatic Ecology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
ACS Omega. 2020 Mar 9;5(10):5241-5248. doi: 10.1021/acsomega.9b04309. eCollection 2020 Mar 17.
The monoterpene thymol has been proposed as a valuable alternative to in-feed antibiotics in animal production. However, the effectiveness of the antimicrobial is comprised by its fast absorption in the upper gastrointestinal tract. In this work, two glucoconjugates, thymol α-d-glucopyranoside (TαG) and thymol β-d-glucopyranoside (TβG), were compared with free thymol for their potential to deliver higher concentrations of the active compound to the distal small intestine of supplemented piglets. Additionally, an analytical method was developed and validated for the simultaneous quantification of thymol and its glucoconjugates in different matrices. In stomach contents of pigs fed with 3333 μmol kg thymol, TαG, or TβG, total thymol concentrations amounted to 3048, 2357, and 1820 μmol kg dry matter, respectively. In glucoconjugate-fed pigs, over 30% of this concentration was present in the unconjugated form, suggesting partial hydrolysis in the stomach. No quantifiable levels of thymol or glucoconjugates were detected in the small intestine or cecum for any treatment, indicating that conjugation with one glucose unit did not sufficiently protect thymol from early absorption.
单萜类化合物百里香酚已被提议作为动物生产中饲料抗生素的一种有价值的替代品。然而，这种抗菌剂的有效性因其在上消化道的快速吸收而受到影响。在这项研究中，将两种葡糖共轭物，百里香酚α - d - 吡喃葡萄糖苷（TαG）和百里香酚β - d - 吡喃葡萄糖苷（TβG）与游离百里香酚进行比较，看它们能否将更高浓度的活性化合物输送到补充了这些物质的仔猪的远端小肠。此外，还开发并验证了一种分析方法，用于同时定量不同基质中百里香酚及其葡糖共轭物。在喂食了3333 μmol/kg百里香酚、TαG或TβG的猪的胃内容物中，百里香酚的总浓度分别为3048、2357和1820 μmol/kg干物质。在喂食葡糖共轭物的猪中，该浓度的30%以上以未共轭形式存在，这表明在胃中发生了部分水解。对于任何处理，在小肠或盲肠中均未检测到可量化水平的百里香酚或葡糖共轭物，这表明与一个葡萄糖单元结合并不能充分保护百里香酚不被早期吸收。
