Mehdizadeh Robab, Akmali Vahid, Sharifi Mozafar
Department of Biology, Faculty of Science, Razi University, Baghabrisham, Kermanshah, Iran.
Mitochondrial DNA A DNA Mapp Seq Anal. 2020 Apr;31(3):87-97. doi: 10.1080/24701394.2020.1741562. Epub 2020 Mar 22.
In this paper, we investigate the genetic structure and phylogeography of , using the mitochondrial cytochrome b gene (1017 bp) in Iran and adjacent regions. The total haplotype and nucleotide diversity are 0.63 ± 0.055 and 0.0021 ± 0.00017, respectively which suggest that exhibits low genetic diversity. AMOVA analysis shows that more variation of genetic differentiation is present among populations of phylogenetic groupings than within populations. Our phylogenetic results support the monophyly of and suggest this taxon comprises three allopatric/parapatric phylogroups that are distributed in Europe-western Turkey, eastern Turkey-northern Iran, and southern Iran. The Europe-western Turkey lineage (clade 2) split from the eastern Turkey-Iran lineage (clade 1) during the middle Pleistocene (0.8534 (ca.I)-0.6454 (ca.II) Ma). The divergence time among subclades A and B occurred during the mid-Pleistocene (0.4849 (ca.I)-0.369 (ca.II) Ma). All phylogenetic analyses also indicate that the Iranian and eastern Turkey diverged from Europe and western Turkey , with the mean percentage sequence differences ranging from 0.92%-0.75% between them. We infer that long-term isolation of in spatially distinct refugia in parts of southwestern and northeastern Iran has promoted distinct phylogeographic lineages during the Pleistocene.
在本文中，我们利用线粒体细胞色素b基因（1017 bp）对伊朗及周边地区的[物种名称未给出]的遗传结构和系统地理学进行了研究。总的单倍型多样性和核苷酸多样性分别为0.63±0.055和0.0021±0.00017，这表明[物种名称未给出]具有较低的遗传多样性。分子方差分析（AMOVA）表明，系统发育分组群体间的遗传分化变异比群体内更多。我们的系统发育结果支持[物种名称未给出]的单系性，并表明该分类单元包括三个异域/邻域分布的系统发育组，分别分布在欧洲 - 土耳其西部、土耳其东部 - 伊朗北部和伊朗南部。欧洲 - 土耳其西部谱系（分支2）在中更新世（0.8534（约I） - 0.6454（约II）百万年前）从土耳其东部 - 伊朗谱系（分支1）分化出来。亚分支A和B之间的分歧时间发生在中更新世（0.4849（约I） - 0.369（约II）百万年前）。所有系统发育分析还表明，伊朗和土耳其东部的[物种名称未给出]与欧洲和土耳其西部的[物种名称未给出]分化，它们之间的平均序列差异百分比在0.92% - 0.75%之间。我们推断，更新世期间，[物种名称未给出]在伊朗西南部和东北部部分地区空间上不同的避难所中长期隔离，促进了不同的系统地理谱系的形成。
