Artelt P, Morelle C, Ausmeier M, Fitzek M, Hauser H
Cell Biology and Genetics Section, GBF-Gesellschaft für Biotechnologische Forschung mbH., Braunschweig, (F.R.G.).
Gene. 1988 Sep 7;68(2):213-9. doi: 10.1016/0378-1119(88)90023-6.
We have constructed two related types of multi-cloning mammalian expression vectors. The first, pMPSVEH/HE, carries the promoter of the myeloproliferative sarcoma virus (MPSV). This promoter was found to be stronger than both the SV40 early and the trans-activated human immunodeficiency virus promoters in many cell lines including human and rodent fibroblastoid, lymphoid or myeloid cells. The other, pBEH/HE, carries the simian virus 40 (SV40) early promoter and origin of replication. This offers the possibility of encapsidation in SV40 pseudovirions and subsequent gene transfer into, e.g., hemopoietic cells, via infection. The usefulness of the expression systems was tested with a number of genes and cell lines.
我们构建了两种相关类型的多克隆哺乳动物表达载体。第一种是pMPSVEH/HE，携带骨髓增殖性肉瘤病毒（MPSV）的启动子。在包括人和啮齿类成纤维细胞样、淋巴样或髓样细胞在内的许多细胞系中，发现该启动子比SV40早期启动子和反式激活的人类免疫缺陷病毒启动子都更强。另一种是pBEH/HE，携带猿猴病毒40（SV40）早期启动子和复制起点。这提供了被包装到SV40假病毒颗粒中并随后通过感染将基因转移到例如造血细胞中的可能性。使用许多基因和细胞系对这些表达系统的实用性进行了测试。
