Shih A P, Zbuzek V K, Sauchelli F C, Wu W H
UMD-New Jersey Medical School, Department of Anesthesiology, Newark.
Gerontology. 1988;34(5-6):257-63. doi: 10.1159/000212964.
The effect of aging on vasopressin (VP) clearance was studied in male Fischer 344 rats 2 (young), 12 (adult) and 30 (old) months of age using in situ liver perfusion technique. The liver was perfused with Krebs-Henseleit bicarbonate buffer containing 1% bovine serum albumin and VP (2 ng/ml), at a constant temperature of 37 +/- 0.5 degrees C and a flow rate of 150 ml/h for 120 min. Expressed per whole liver and per gram tissue, VP declined exponentially over the studied period fitting well to the curve y = bekx (p = 0.0001) in all three groups. The analysis of covariance revealed the slope of the VP decay curve to be steepest in old rats, significantly different from that of young (p = 0.0001 expressed per whole liver or per gram tissue) and adult (p = 0.0001 and p = 0.001, expressed per whole liver and per gram tissue, respectively) rats. This suggests that, at least in male Fischer 344 rats, the hepatic clearance of VP was fastest in old rats.
采用原位肝脏灌注技术，对2个月（年轻）、12个月（成年）和30个月（老年）的雄性Fischer 344大鼠研究衰老对血管加压素（VP）清除率的影响。在37±0.5℃恒温、流速150 ml/h条件下，用含1%牛血清白蛋白和VP（2 ng/ml）的Krebs-Henseleit碳酸氢盐缓冲液对肝脏灌注120分钟。以全肝和每克组织表示，在研究期间VP呈指数下降，在所有三组中均很好地拟合曲线y = bekx（p = 0.0001）。协方差分析显示，老年大鼠VP衰减曲线的斜率最陡，与年轻大鼠（以全肝或每克组织表示，p = 0.0001）和成年大鼠（分别以全肝和每克组织表示，p = 0.0001和p = 0.001）的斜率有显著差异。这表明，至少在雄性Fischer 344大鼠中，老年大鼠肝脏对VP的清除最快。
