Department of Geriatrics and Neurology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China; Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Chemosphere. 2020 Aug;252:126510. doi: 10.1016/j.chemosphere.2020.126510. Epub 2020 Mar 16.
The present study aimed to identify the effects of arsenic on behaviors in Caenorhabditis elegans (C. elegans) and the transgenerational effects. The synchronized C. elegans (P generation) were exposed to 0, 0.2, 1.0, and 5.0 mM NaAsO and the subsequent generations (F1 and F2) were maintained on fresh nematode growth medium (NGM). The behaviors and growth were recorded at 0, 12, 24, 36, 48, 60, and 72 h post synchronization. The results demonstrated that arsenic affected various indicators regarding the behavior (head thrash, body bend, movement speed, wavelength, amplitude and so on) and in general the effects started to accumulate from 24 h and lasted throughout the exposure. The behavior impairments were transgenerational with varying patterns, amongst the head thrash and body bend responded most sensitively though the responses gradually declined across generations. Arsenic exposure inhibited the growth (body length, body width, and body area) in P C. elegans from 24 h to 60 h, however there was no difference between treatments groups and the control at 72 h. Arsenic led to a dose-dependent degeneration of dopaminergic neurons in C. elegans, and inhibition of BAS-1 and CAT-2 expressions. The expressions of GCS-1, GSS-1, and SKN-1 were induced by arsenic exposure. Overall, chronic arsenic exposure impaired the behaviors and there were transgenerational effects. The head thrash and body bend responded most sensitively. Arsenic induced behavioral disorders might be attributed to degeneration of dopaminergic neurons which was associated with oxidative stress.
本研究旨在探讨砷对秀丽隐杆线虫（C. elegans）行为的影响及其跨代效应。将同步化的 C. elegans（P 代）暴露于 0、0.2、1.0 和 5.0 mM NaAsO 中，随后的世代（F1 和 F2）在新鲜线虫生长培养基（NGM）中维持。在同步后 0、12、24、36、48、60 和 72 h 记录行为和生长情况。结果表明，砷对行为的多个指标（头部摆动、身体弯曲、运动速度、波长、振幅等）产生影响，一般来说，从 24 h 开始，这些影响开始积累并持续整个暴露期。行为损伤具有跨代效应，表现形式各异，其中头部摆动和身体弯曲反应最为敏感，但随着世代的推移，反应逐渐减弱。砷暴露从 24 h 到 60 h 抑制了 P 代 C. elegans 的生长（体长、体宽和体面积），但在 72 h 时，处理组与对照组之间没有差异。砷导致 C. elegans 中的多巴胺能神经元出现剂量依赖性退化，并抑制了 BAS-1 和 CAT-2 的表达。砷暴露诱导了 GCS-1、GSS-1 和 SKN-1 的表达。总的来说，慢性砷暴露会损害行为，并产生跨代效应。头部摆动和身体弯曲反应最为敏感。砷引起的行为障碍可能归因于多巴胺能神经元的退化，这与氧化应激有关。
