Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, People's Republic of China; Jiangsu Safety Assessment and Research Center for Drug, Pesticide, and Veterinary Drug, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, People's Republic of China.
Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, People's Republic of China.
Toxicol Lett. 2020 Jun 15;326:11-17. doi: 10.1016/j.toxlet.2020.03.002. Epub 2020 Mar 4.
Arsenic (As) is a toxic element that is highly abundant in the environment. However, there has not been sufficient research into the mechanisms of arsenic-induced transgenerational effects. In biomedical and environmental toxicology research field, C. elegans are often used as the ideal model. In this study, F0 generation animals were cultured with arsenite, while subsequent generations animals (F1 - F6) were cultured in the absence of arsenic. Experiments were performed to examine the transgenerational glycometabolism and the associated mechanisms in all seven generations (F0 - F6) of C. elegans. Results show that arsenite exposure increased total glucose content but reduced glucose metabolites in F0 generation C. elegans. The total glucose content was also elevated in subsequent generations probably due to transgenerational downregulation of fgt-1. In addition, arsenite exposure induced transgenerational downregulation of histone demethyltransferase spr-5 and elevated histone dimethylation in F0 generation. This study highlights that single generation exposure to arsenite causes transgenerational changes in glycometabolism in C. elegans, which may be caused by downregulation of spr-5 and elevation of H3K4me2.
砷（As）是一种在环境中含量很高的有毒元素。然而，对于砷诱导的跨代效应的机制，还没有足够的研究。在生物医学和环境毒理学研究领域，秀丽隐杆线虫通常被用作理想的模型。在这项研究中，F0 代动物用亚砷酸盐培养，而随后的几代动物（F1-F6）在没有砷的情况下培养。实验研究了所有 7 代（F0-F6）秀丽隐杆线虫的跨代糖代谢及其相关机制。结果表明，亚砷酸盐暴露增加了 F0 代秀丽隐杆线虫的总葡萄糖含量，但降低了葡萄糖代谢物。由于 fgt-1 的跨代下调，随后几代的总葡萄糖含量也升高。此外，亚砷酸盐暴露诱导 F0 代组蛋白去甲基化酶 spr-5 的跨代下调，并导致 H3K4me2 水平升高。这项研究强调，单代暴露于亚砷酸盐会导致秀丽隐杆线虫的糖代谢发生跨代变化，这可能是由 spr-5 的下调和 H3K4me2 的升高引起的。
