一种聚阴离子（戊聚糖多硫酸酯）的抗补体活性，II.——作用方式及对人补体溶血活性的“体外”抑制（作者译）

[Anticomplementary activity of a polyanion: pentosan-poly-sulfoester, II.--Mode of action and "in vitro " inhibition of human complement hemolytic activity (author's transl)].

作者信息

Berthoux F X, Freyria A M, Traeger J

出版信息

Pathol Biol (Paris). 1977 Feb;25(2):105-8.

PMID:322041

Abstract

The drug, pentosan-poly-sulfoester (PPS), is a potent in vitro inhibitor of the human complement hemolytic activity. This CH 50 inhibition represents a real anticomplementary activity (ACA), because this drug has no effect on sensitized sheep red blood cells (EA). The inhibition curve of human serum CH 50, by PPS is sigmoidal. The 50% inhibition is obtained for a 1: 650 dilution of PPS, which corresponds to a concentration of 0.08 mg/ml in normal human serum. Hemolytic titrations of C1, C4, C2, C3, and C5 showed a complete inhibition of C4, C2 and C3, and a partial inhibition of C1 (C1q, C1r, C1s, Ca++) and C5, by this drug. The mechanism of such functional inactivation of the different complement components is not yet elucidated.

摘要

药物戊聚糖多硫酸酯（PPS）是一种强大的体外人补体溶血活性抑制剂。这种对CH50的抑制代表了真正的抗补体活性（ACA），因为该药物对致敏绵羊红细胞（EA）没有作用。PPS对人血清CH50的抑制曲线呈S形。PPS在1:650稀释时可获得50%的抑制率，这相当于正常人血清中浓度为0.08mg/ml。对C1、C4、C2、C3和C5的溶血滴定显示，该药物可完全抑制C4、C2和C3，并部分抑制C1（C1q、C1r、C1s、Ca++）和C5。不同补体成分这种功能失活的机制尚未阐明。