Two-dimensional hydrophilic interaction and reversed phase liquid chromatography easily extracted pesticides and polar pesticides multi-residue method--A concept.

We offer a concept for the simultaneous determination of QuEChERS amenable and highly polar pesticides using two-dimensional liquid chromatography high resolution mass spectrometry with electrospray ionization (ESI) and polarity switching. First results proved feasibility to determine glyphosate, AMPA, ethephon, glufosinate, MPPA, fosetyl-Al, N-acetyl-glufosinate, N-acetyl-glyphosate and N-acetyl-AMPA including fragments, next to 325 QuEChERS amenable pesticides. We plan to investigate an efficient extraction procedure by either diluting with water or acetonitrile extraction in mini scale without the need for additional clean-up steps. After completion of all experiments, the method will be validated and expanded for further pesticides and organic contaminants. Therefore, we present a concept for a Two-dimensional HILIC Reversed phase Easily Extracted Pesticides and Polar Pesticides (THREEP or P) multi-residue method.