MCCC2 过表达预示结直肠癌预后不良，并促进细胞增殖。

MCCC2 overexpression predicts poorer prognosis and promotes cell proliferation in colorectal cancer.

机构信息

Department of BIN Convergence Technology and Polymer Nano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Republic of Korea; Fourth Ward of Medical Care Center, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.

Department of BIN Convergence Technology and Polymer Nano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Republic of Korea.

出版信息

Exp Mol Pathol. 2020 Aug;115:104428. doi: 10.1016/j.yexmp.2020.104428. Epub 2020 Mar 20.

DOI:10.1016/j.yexmp.2020.104428

PMID:32205097

Abstract

PURPOSES

Recently, Methylcrotonoyl-CoA carboxylase 2 (MCCC2) is reported to be involved in tumor formation and progression. However, MCCC2 has nerve been reported in colorectal cancer. In this study, we aimed to investigate the role of MCCC2 in colorectal cancer.

METHODS

118 colorectal cancer and matched adjacent normal tissues were enrolled in this study. The expression level of MCCC2 was measured by quantificational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). The clinical significance of MCCC2 and its influence on cell proliferation was further analyzed.

RESULTS

Results shown that the mRNA levels of MCCC2 in colorectal cancer tissues were significantly increased compared with those in normal tissues (P < .0001). MCCC2 high-expression was observed in 56.8% colorectal cancer tissues, which was significantly higher than those in normal controls (9.3%, P < .0001). MCCC2 high-expression correlated with tumor size, T stage, lymph node metastasis, distant metastasis, clinical stage and differentiation in colorectal cancer (P < .05). Moreover, MCCC2 high-expression predicted poorer prognosis and could be as an independent prognostic factor. In addition, MCCC2 knockdown significantly inhibited cell proliferation compared with these controls, while MCCC2 overexpression could reverse the effect.

CONCLUSION

These data indicate MCCC2 overexpression promotes cell proliferation and predicts poorer prognosis in colorectal cancer.

摘要

目的

最近有研究报道甲基巴豆酰辅酶 A 羧化酶 2（MCCC2）参与肿瘤的形成和进展。然而，MCCC2 在结直肠癌中的作用尚未见报道。本研究旨在探讨 MCCC2 在结直肠癌中的作用。

方法

本研究纳入了 118 例结直肠癌及配对的癌旁正常组织。采用实时定量聚合酶链反应（qRT-PCR）和免疫组织化学（IHC）检测 MCCC2 的表达水平。进一步分析 MCCC2 的临床意义及其对细胞增殖的影响。

结果

结果显示，结直肠癌组织中 MCCC2 的 mRNA 水平明显高于正常组织（P<0.0001）。56.8%的结直肠癌组织中存在 MCCC2 高表达，明显高于正常对照组（9.3%，P<0.0001）。MCCC2 高表达与结直肠癌的肿瘤大小、T 分期、淋巴结转移、远处转移、临床分期和分化有关（P<0.05）。此外，MCCC2 高表达预示着更差的预后，并且可以作为独立的预后因素。此外，与对照组相比，MCCC2 敲低显著抑制细胞增殖，而 MCCC2 过表达可逆转这种作用。