Department of Zoology, University of Delhi, Delhi, 110007, India.
Department of Zoology, University of Lucknow, Lucknow, 226007, India.
Mol Cell Endocrinol. 2020 May 15;508:110794. doi: 10.1016/j.mce.2020.110794. Epub 2020 Mar 20.
We investigated gonadal effects on hypothalamic transcription of genes in sham-operated and castrated redheaded buntings photostimulated into spring and autumn migratory states. RNA-Seq results showed testes-dependent differences between spring and autumn migratory states. In particular, differentially expressed genes enriched G-protein-coupled receptor and calcium-ion signaling pathways during spring and autumn states, respectively. qPCR assay showed attenuated gabra5, ttr, thra and thrb expressions, suggesting reduced GABA and thyroid hormone effects on photo-sexual response in spring. In spring castrates, reduced npy, tac1 and nrcam and increased ank3 expression suggested testicular effects on the appetite, prolactin release and neuronal functions, whereas in autumn castrates, reduced rasgrp1, grm5 and grin1, and increased mras expression suggested testicular effects on the ras, G-protein and glutamate signaling pathways. Castration-induced reciprocal switching of pomc and pdyn expressions suggested effects on the overall homeostasis in both seasons. These results demonstrate transcriptome-wide changes, with season-dependent roles of testes in songbird migration.
我们研究了性腺对处于春季和秋季迁徙状态的被光刺激的红额金翅雀下丘脑转录基因的影响。RNA-Seq 结果显示，春季和秋季迁徙状态之间存在依赖于睾丸的差异。特别是，差异表达的基因在春季和秋季状态下分别富集了 G 蛋白偶联受体和钙离子信号通路。qPCR 检测显示，gabra5、ttr、thra 和 thrb 的表达减弱，表明春季 GABA 和甲状腺激素对光性反应的影响降低。在春季去势雄鸟中，npy、tac1 和 nrcam 表达减少，ank3 表达增加，表明睾丸对食欲、催乳素释放和神经元功能有影响，而在秋季去势雄鸟中，rasgrp1、grm5 和 grin1 表达减少，mras 表达增加，表明睾丸对 ras、G 蛋白和谷氨酸信号通路有影响。去势诱导的 pomc 和 pdyn 表达的相互转换表明，睾丸在两个季节对整体内稳态都有影响。这些结果表明，转录组发生了广泛的变化，睾丸在鸣禽迁徙中具有季节性作用。
