Van 't Klooster G A, Boot J H, Mennes W C, Blaauboer B J
Institute of Veterinary Pharmacology, Pharmacy and Toxicology, University of Utrecht, The Netherlands.
J Chromatogr. 1988 Nov 18;432:223-31. doi: 10.1016/s0378-4347(00)80647-7.
An ion-pair high-performance liquid chromatographic method for the rapid, selective and sensitive analysis of samples containing bromosulphophthalein (BSP) and its conjugates is presented. The method is useful for analysis in bile, culture media and cultured hepatocytes. Two sample preparation methods are described. Even though BSP recovery from albumin binding is complete, only a small percentage of free BSP can be detected in cells, possibly owing to a conjugation-related pool of BSP in cells. As BSP-glutathione recovery is complete, the method offers a useful tool to investigate impairment of glutathione conjugation.
本文介绍了一种离子对高效液相色谱法，用于快速、选择性和灵敏地分析含有溴磺酞（BSP）及其结合物的样品。该方法可用于胆汁、培养基和培养的肝细胞的分析。描述了两种样品制备方法。尽管从白蛋白结合中回收的BSP是完全的，但在细胞中只能检测到一小部分游离BSP，这可能是由于细胞中存在与结合相关的BSP池。由于BSP-谷胱甘肽的回收是完全的，该方法为研究谷胱甘肽结合受损提供了一个有用的工具。
