高效液相色谱法测定尿液中的乙醛酸

High-performance liquid chromatographic determination of glyoxylic acid in urine.

作者信息

Petrarulo M, Pellegrino S, Bianco O, Marangella M, Linari F, Metasti E

机构信息

Laboratory of Renal Stone Disease, Ospedale Mauriziano Umberto, Turin, Italy.

出版信息

J Chromatogr. 1988 Nov 18;432:37-46. doi: 10.1016/s0378-4347(00)80631-3.

DOI:10.1016/s0378-4347(00)80631-3

PMID:3220907

Abstract

A high-performance liquid chromatographic (HPLC) method for the determination of urinary glyoxylic acid is proposed. The system is based on the precolumn derivatization of alpha-keto acids by means of phenylhydrazine, separation of the phenylhydrazone formed by HPLC and spectrophotometric detection at 324 nm. The method is precise and allows the determination of 0.5 mumol/l glyoxylate. The poor stability of glyoxylate under all conventional preservation conditions requires the analysis to be carried out as soon as possible after urine collection. Results of determinations on urine samples from healthy controls and from patients with idiopathic calcium stone disease and type I primary hyperoxaluria are reported.

摘要

提出了一种测定尿中乙醛酸的高效液相色谱（HPLC）方法。该系统基于通过苯肼对α-酮酸进行柱前衍生化，利用HPLC分离形成的苯腙，并在324nm处进行分光光度检测。该方法精确，可测定0.5μmol/L的乙醛酸。在所有常规保存条件下，乙醛酸稳定性较差，因此需要在收集尿液后尽快进行分析。报告了对健康对照者、特发性钙结石病患者和I型原发性高草酸尿症患者尿液样本的测定结果。