Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), Carretera De la Coruña KM 5.9, 28040, Madrid, Spain; and School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Reprod Fertil Dev. 2020 Mar;32(6):564-571. doi: 10.1071/RD19360.
The aim of this study was to determine the effect of maternal-embryonic asynchrony in the reproductive tract (oviduct and uterus) on subsequent embryo development in cattle. Fifty Day 1invitro-produced zygotes were transferred endoscopically into the oviduct ipsilateral to the corpus luteum of heifers (n=40) that were either synchronous with the embryos (Day 1 after ovulation) or asynchronous and ahead of the embryo (Day 3 after ovulation). A subset of heifers was killed in a commercial abattoir 3, 6 or 14 days after embryo transfer. Location within the reproductive tract, developmental stage and the quality of embryos were recorded. Transfer of embryos to an advanced (asynchronous) oviduct resulted, on Day 4, in fewer embryos at the expected location (oviduct), and a greater number of degenerated and retarded embryos with a lower total cell number than for embryos in the synchronous group. Similarly, on Day 7, asynchrony led to a greater number of degenerated and retarded embryos compared with the synchronous group. Total embryo cell number was similar among groups. Although Day 15 conceptuses were longer following asynchronous transfer, only 50% of the asynchronous heifers yielded conceptuses, compared with 100% in the synchronous group. In conclusion, asynchrony between the developing embryo and the reproductive tract has a negative effect on embryo development.
本研究旨在确定生殖道（输卵管和子宫）中母体-胚胎的不同步对牛随后胚胎发育的影响。将 50 枚第 1 天体外生产的胚胎通过内窥镜转移到黄体同侧的输卵管中（n=40），这些胚胎与胚胎同步（排卵后第 1 天）或不同步且提前于胚胎（排卵后第 3 天）。一小部分奶牛在胚胎移植后 3、6 或 14 天在商业屠宰场被杀死。记录胚胎在生殖道内的位置、发育阶段和胚胎质量。将胚胎转移到提前（不同步）的输卵管中，在第 4 天导致较少的胚胎处于预期位置（输卵管），并且退化和发育迟缓的胚胎数量增加，总细胞数低于同步组。同样，在第 7 天，与同步组相比，不同步导致更多的退化和发育迟缓的胚胎。胚胎总细胞数在各组之间相似。尽管在异步转移后第 15 天的胚胎更长，但只有 50%的异步奶牛产生胚胎，而同步组为 100%。总之，胚胎发育与生殖道的不同步对胚胎发育有负面影响。
