van Kooij R J, te Velde E R
Department of Obstetrics and Gynaecology, University Hospital, Utrecht, The Netherlands.
Hum Reprod. 1988 Aug;3(6):773-5. doi: 10.1093/oxfordjournals.humrep.a136782.
Human zonae pellucidae were added to suspensions of capacitating spermatozoa. The zonae were prepared from oocytes that remained unfertilized in our in-vitro fertilization (IVF) programme. After capacitation, zona free hamster oocytes were added and the penetration rate of the hamster oocytes was determined. Controls without the addition of zonae were run in parallel. A distinct enhancement of the penetration rate was found when zonae were present during capacitation, using sperm of fertile donors. Sperm of several infertile men did not respond to the presence of zonae with higher penetration rates. It was concluded that the zona pellucida is involved in the induction of the acrosome-reaction of human spermatozoa. In addition the incubation system with zonae as described might be useful for the identification of infertile semen.
将人透明带添加到获能精子悬液中。透明带取自我们体外受精（IVF）项目中未受精的卵母细胞。获能后，加入无透明带的仓鼠卵母细胞并测定仓鼠卵母细胞的穿透率。同时进行不添加透明带的对照实验。当使用可育供体的精子在获能过程中存在透明带时，发现穿透率有明显提高。几名不育男性的精子对透明带的存在没有产生更高穿透率的反应。得出的结论是，透明带参与了人类精子顶体反应的诱导。此外，所述的含透明带孵育系统可能有助于不育精液的鉴定。
