Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University.
Department of Urology, the Second Affiliated Hospital of Xi'an Jiaotong University.
Anticancer Drugs. 2020 Sep;31(8):785-791. doi: 10.1097/CAD.0000000000000931.
Silibinin is a flavonoid extract isolated from milk thistle and has been proved to be a promising chemotherapeutic drug for cancer. However, most of those studies were performed on the human cancer cells, where the effects of silibinin could only be observed on an animal model with a deficient immune system. RenCa cells were isolated from a murine spontaneous renal cell carcinoma, which resembles many features of human renal cell carcinoma, and have been used to establish animal models with a sound immune response. Herein, we investigated the anti-cancer effects of silibinin on RenCa cells, revealing that it inhibited cell viability in both dose- and time-dependent manners. Silibinin slightly triggered apoptosis and significantly induced G2-M cell cycle arrest by downregulating cyclin B1 and CDK1 and increasing expression of p21. Furthermore, silibinin significantly inhibited the growth of RenCa cell xenografts in vivo. In addition, we found that silibinin reduced programmed cell death 1 ligand 1 expression of RenCa cells in vivo and in vitro. Our findings demonstrate that silibinin can inhibit the growth of mouse tumor cells in an animal model with an intact immune system, and silibinin may decrease the immunosuppression effect of tumor cells. Our results provide new evidence for evaluation of Silibinin application in cancer therapy.
水飞蓟宾是从奶蓟草中分离得到的一种黄酮类提取物，已被证明是一种很有前途的癌症化疗药物。然而，大多数这些研究都是在人类癌细胞上进行的，在这些研究中，只有在免疫系统缺陷的动物模型上才能观察到水飞蓟宾的效果。RenCa 细胞是从鼠自发性肾细胞癌中分离出来的，它与许多人类肾细胞癌的特征相似，并被用于建立具有健全免疫反应的动物模型。在此，我们研究了水飞蓟宾对 RenCa 细胞的抗癌作用，结果表明它以剂量和时间依赖的方式抑制细胞活力。水飞蓟宾轻微触发细胞凋亡，通过下调细胞周期蛋白 B1 和 CDK1 并增加 p21 的表达，显著诱导 G2-M 细胞周期阻滞。此外，水飞蓟宾显著抑制 RenCa 细胞异种移植瘤在体内的生长。此外，我们发现水飞蓟宾在体内和体外降低了 RenCa 细胞程序性细胞死亡配体 1 的表达。我们的研究结果表明，水飞蓟宾可以抑制具有完整免疫系统的动物模型中鼠肿瘤细胞的生长，水飞蓟宾可能会降低肿瘤细胞的免疫抑制作用。我们的结果为评估水飞蓟宾在癌症治疗中的应用提供了新的证据。
