School of Medicine, Huaqiao University, Quanzhou 362021, Fujian, China.
Int J Mol Sci. 2020 Mar 23;21(6):2211. doi: 10.3390/ijms21062211.
The compound 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (-DDT) has been identified as one of the endocrine-disrupting chemicals causing adverse effects on wildlife and even humans through bioaccumulation. Its detection has become increasingly important. We have obtained candidate aptamers binding to '-DDT by a systematic evolution of ligands by exponential enrichment (SELEX) protocol. Five out of seventeen candidate sequences were selected for preliminary characterization by SYBR Green I assay. One sequence with highest fluorescence response with -DDT, designated DDT_13, was chosen for further characterization. Its dissociation constant (K) was determined to be 412.3 ± 124.6 nM. DDT_13 exhibited low cross-binding activities on other tested small molecules. The good bioactivities of DDT_13 were demonstrated for the analysis of spiked lake water and tap water samples. This study provides a novel -DDT-specific probe for its future applications.
1,1,1-三氯-2-(对氯苯基)-2-(邻氯苯基)乙烷（-DDT）已被确定为一种内分泌干扰化学物质，通过生物积累对野生动物甚至人类造成不良影响。因此，对其的检测变得越来越重要。我们通过指数富集的配体系统进化（SELEX）方案获得了与 -DDT 结合的候选适体。从十七个候选序列中选择了五个进行 SYBR Green I 测定的初步特征分析。选择与 -DDT 具有最高荧光响应的一个序列，命名为 DDT_13，用于进一步表征。其解离常数（K）确定为 412.3 ± 124.6 nM。DDT_13 对其他测试的小分子表现出低的交叉结合活性。DDT_13 在分析加标湖水和自来水样品方面表现出良好的生物活性。本研究为其未来的应用提供了一种新型的 -DDT 特异性探针。
