N-琥珀酰-丙氨酰-甲硫氨酰-S-苄基半胱氨酸对硝基苯胺作为测定半胱氨酸蛋白酶活性的灵敏底物。
N-succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide as a sensitive substrate for assaying activity of cysteine proteinases.
作者信息
Fukal L, Kasafírek E
机构信息
Department of Physiology, University of Agriculture, Prague, Czechoslovakia.
出版信息
J Biochem Biophys Methods. 1988 Aug;16(4):323-5. doi: 10.1016/0165-022x(88)90067-x.
N-Succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide has been found to be a very sensitive chromogenic substrate for the assay of cysteine proteinase papain, ficin and bromelain. N-Succinyl-alanyl-S-benzylcysteine p-nitroanilide and N-succinyl-alanyl-alanyl-S-benzylcysteine p-nitroanilide are also suitable for this purpose. These substrates were hydrolyzed only very slightly or not hydrolyzed at all by trypsin.
已发现N-琥珀酰丙氨酰-甲硫氨酰-S-苄基半胱氨酸对硝基苯胺是用于测定半胱氨酸蛋白酶木瓜蛋白酶、无花果蛋白酶和菠萝蛋白酶的一种非常灵敏的显色底物。N-琥珀酰丙氨酰-S-苄基半胱氨酸对硝基苯胺和N-琥珀酰丙氨酰丙氨酰-S-苄基半胱氨酸对硝基苯胺也适用于此目的。这些底物仅被胰蛋白酶轻微水解或根本不被水解。
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