Teo Guan-Young, Rasedee Abdullah, Al-Haj Nagi A, Beh Chaw Yee, How Chee Wun, Rahman Heshu Sulaiman, Alitheen Noorjahan Banu, Rosli Rozita, Abdullah Al-Shwyeh Hussah, Ali Abdelwahid Saeed
Institute of Bioscience, Universiti Putra Malaysia, Malaysia.
Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia.
Saudi J Biol Sci. 2020 Feb;27(2):653-658. doi: 10.1016/j.sjbs.2019.11.032. Epub 2019 Dec 6.
Erythropoietin receptors (EPORs) are present not only in erythrocyte precursors but also in non-hematopoietic cells including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in culture medium on the EPOR expression and viability of the estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells. Using flow cytometry, we showed that the inclusion of 10% FBS in the medium increased the EPOR expressions and viabilities of MDA-MB-231 and MCF-7 cells. The MDA-MB-231 showed greater EPOR expression than MCF-7 cells, suggesting that the presence of ERs on cells is associated with poor expression of EPOR. Culture medium containing 10% FBS also caused increased number of breast cancer cells entering the synthesis phase of the cell cycle. The study also showed that rHuEPO treatment did not affect viability of breast cancer cells. In conclusion, it was shown that the inclusion of FBS in culture medium increased expression of EPOR in breast cancer cells and rHuEPO treatment had no effect on the proliferation of these cancer cells.
促红细胞生成素受体（EPORs）不仅存在于红细胞前体中，还存在于包括癌细胞在内的非造血细胞中。在本研究中，我们测定了培养基中的胎牛血清（FBS）对雌激素受体（ER）阳性的MCF-7和ER阴性的MDA-MB-231乳腺癌细胞的EPOR表达及活力的影响。通过流式细胞术，我们发现培养基中加入10% FBS可增加MDA-MB-231和MCF-7细胞的EPOR表达及活力。MDA-MB-231细胞的EPOR表达高于MCF-7细胞，这表明细胞上ER的存在与EPOR的低表达有关。含有10% FBS的培养基还导致进入细胞周期合成期的乳腺癌细胞数量增加。该研究还表明，重组人促红细胞生成素（rHuEPO）处理不影响乳腺癌细胞的活力。总之，研究表明培养基中加入FBS可增加乳腺癌细胞中EPOR的表达，而rHuEPO处理对这些癌细胞的增殖没有影响。
