• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Characterization of the transcriptomes of -induced hair cells in the mouse cochlea.小鼠耳蜗中诱导毛细胞转录组的特征分析。
Am J Stem Cells. 2020 Feb 15;9(1):1-15. eCollection 2020.
2
Generation of mature and functional hair cells by co-expression of Gfi1, Pou4f3, and Atoh1 in the postnatal mouse cochlea.在出生后小鼠耳蜗中共同表达 Gfi1、Pou4f3 和 Atoh1 生成成熟且功能正常的毛细胞。
Cell Rep. 2021 Apr 20;35(3):109016. doi: 10.1016/j.celrep.2021.109016.
3
Combinatorial Atoh1 and Gfi1 induction enhances hair cell regeneration in the adult cochlea.组合性 Atoh1 和 Gfi1 诱导增强成年耳蜗毛细胞再生。
Sci Rep. 2020 Dec 8;10(1):21397. doi: 10.1038/s41598-020-78167-8.
4
AAV-mediated Gene Cocktails Enhance Supporting Cell Reprogramming and Hair Cell Regeneration.AAV 介导的基因鸡尾酒增强支持细胞的重编程和毛细胞再生。
Adv Sci (Weinh). 2024 Aug;11(29):e2304551. doi: 10.1002/advs.202304551. Epub 2024 May 29.
5
The proper timing of Atoh1 expression is pivotal for hair cell subtype differentiation and the establishment of inner ear function.Atoh1 表达的适当时间对于毛细胞亚型分化和内耳功能的建立至关重要。
Cell Mol Life Sci. 2023 Nov 6;80(12):349. doi: 10.1007/s00018-023-04947-w.
6
In Vivo Cochlear Hair Cell Generation and Survival by Coactivation of β-Catenin and Atoh1.通过β-连环蛋白和Atoh1的共激活在体内产生和存活耳蜗毛细胞
J Neurosci. 2015 Jul 29;35(30):10786-98. doi: 10.1523/JNEUROSCI.0967-15.2015.
7
The role of Espin in the stereocilia regeneration and protection in Atoh1-overexpressed cochlear epithelium.Espin 在 Atoh1 过表达耳蜗上皮细胞中的静纤毛再生和保护中的作用。
Cell Prolif. 2023 Nov;56(11):e13483. doi: 10.1111/cpr.13483. Epub 2023 Apr 21.
8
In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression.通过异位表达Atoh1在新生小鼠耳蜗中体内生成未成熟的内毛细胞。
PLoS One. 2014 Feb 20;9(2):e89377. doi: 10.1371/journal.pone.0089377. eCollection 2014.
9
Extensive Supporting Cell Proliferation and Mitotic Hair Cell Generation by In Vivo Genetic Reprogramming in the Neonatal Mouse Cochlea.新生小鼠耳蜗体内基因重编程诱导支持细胞广泛增殖和有丝分裂毛细胞生成
J Neurosci. 2016 Aug 17;36(33):8734-45. doi: 10.1523/JNEUROSCI.0060-16.2016.
10
Spontaneous Hair Cell Regeneration Is Prevented by Increased Notch Signaling in Supporting Cells.支持细胞中Notch信号增加会阻止毛细胞的自发再生。
Front Cell Neurosci. 2018 May 4;12:120. doi: 10.3389/fncel.2018.00120. eCollection 2018.

引用本文的文献

1
Comparison of GWAS results between de novo tinnitus and cancer treatment-related tinnitus suggests distinctive roles for genetic risk factors.GWAS 结果在新发耳鸣与癌症治疗相关性耳鸣之间的比较表明遗传风险因素的独特作用。
Sci Rep. 2024 Nov 14;14(1):27952. doi: 10.1038/s41598-024-78274-w.
2
Reprogramming with , , and promotes hair cell regeneration in the adult organ of Corti.使用 、 和 进行重编程可促进成年柯蒂氏器中的毛细胞再生。
PNAS Nexus. 2024 Oct 4;3(10):pgae445. doi: 10.1093/pnasnexus/pgae445. eCollection 2024 Oct.
3
Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium.雏鸡听觉上皮中支持细胞逐步向感觉毛细胞的命运转变。
iScience. 2023 Jan 25;26(2):106046. doi: 10.1016/j.isci.2023.106046. eCollection 2023 Feb 17.
4
TUB and ZNF532 Promote the Atoh1-Mediated Hair Cell Regeneration in Mouse Cochleae.TUB和ZNF532促进小鼠耳蜗中Atoh1介导的毛细胞再生。
Front Cell Neurosci. 2021 Nov 8;15:759223. doi: 10.3389/fncel.2021.759223. eCollection 2021.
5
How Zebrafish Can Drive the Future of Genetic-based Hearing and Balance Research.斑马鱼如何推动基于遗传的听力和平衡研究的未来。
J Assoc Res Otolaryngol. 2021 Jun;22(3):215-235. doi: 10.1007/s10162-021-00798-z. Epub 2021 Apr 28.

本文引用的文献

1
High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor.高分辨率转录组学解析体内 Atoh1 介导的成熟耳蜗毛细胞转分化，鉴定出 Isl1 为共同重编程因子。
PLoS Genet. 2018 Jul 31;14(7):e1007552. doi: 10.1371/journal.pgen.1007552. eCollection 2018 Jul.
2
The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex.组蛋白去甲基化酶LSD1通过与Pax2和NuRD阻遏复合物相互作用来调节内耳祖细胞的分化。
PLoS One. 2018 Jan 25;13(1):e0191689. doi: 10.1371/journal.pone.0191689. eCollection 2018.
3
Hedgehog Signaling Promotes the Proliferation and Subsequent Hair Cell Formation of Progenitor Cells in the Neonatal Mouse Cochlea.刺猬信号通路促进新生小鼠耳蜗中祖细胞的增殖及随后的毛细胞形成。
Front Mol Neurosci. 2017 Dec 21;10:426. doi: 10.3389/fnmol.2017.00426. eCollection 2017.
4
Otoferlin acts as a Ca sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.耳毛细胞带状突触中，otoferlin 作为囊泡融合和囊泡池补充的 Ca 传感器发挥作用。
Elife. 2017 Nov 7;6:e31013. doi: 10.7554/eLife.31013.
5
Claudin expression in the rat endolymphatic duct and sac - first insights into regulation of the paracellular barrier by vasopressin.水通道蛋白在大鼠内淋巴管和囊中的表达——血管加压素对细胞旁屏障调节的初步认识。
Sci Rep. 2017 Apr 4;7:45482. doi: 10.1038/srep45482.
6
Atoh1 as a Coordinator of Sensory Hair Cell Development and Regeneration in the Cochlea.Atoh1作为耳蜗中感觉毛细胞发育和再生的协调因子。
Chonnam Med J. 2017 Jan;53(1):37-46. doi: 10.4068/cmj.2017.53.1.37. Epub 2017 Jan 25.
7
Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.耳蜗和前庭器官的祖细胞向内耳细胞类型分化的独特能力。
Development. 2016 Dec 1;143(23):4381-4393. doi: 10.1242/dev.139840. Epub 2016 Oct 27.
8
Extensive Supporting Cell Proliferation and Mitotic Hair Cell Generation by In Vivo Genetic Reprogramming in the Neonatal Mouse Cochlea.新生小鼠耳蜗体内基因重编程诱导支持细胞广泛增殖和有丝分裂毛细胞生成
J Neurosci. 2016 Aug 17;36(33):8734-45. doi: 10.1523/JNEUROSCI.0060-16.2016.
9
Role of Wnt and Notch signaling in regulating hair cell regeneration in the cochlea.Wnt和Notch信号通路在调控耳蜗毛细胞再生中的作用。
Front Med. 2016 Sep;10(3):237-49. doi: 10.1007/s11684-016-0464-9. Epub 2016 Sep 7.
10
Co-regulation of the Notch and Wnt signaling pathways promotes supporting cell proliferation and hair cell regeneration in mouse utricles.Notch 和 Wnt 信号通路的共调控促进小鼠耳石中支持细胞的增殖和毛细胞的再生。
Sci Rep. 2016 Jul 20;6:29418. doi: 10.1038/srep29418.

小鼠耳蜗中诱导毛细胞转录组的特征分析。

Characterization of the transcriptomes of -induced hair cells in the mouse cochlea.

作者信息

Liu Li-Man, Zhao Li-Ping, Wu Ling-Jie, Guo Luo, Li Wen-Yan, Chen Yan

机构信息

ENT Institute and Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University Shanghai 200031, China.

NHC Key Laboratory of Hearing Medicine (Fudan University) Shanghai 200031, China.

出版信息

Am J Stem Cells. 2020 Feb 15;9(1):1-15. eCollection 2020.

PMID:32211215
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7076321/
Abstract

Postnatal mammalian cochlear hair cells (HCs) can be regenerated by direct transdifferentiation or by mitotic regeneration from supporting cells through many pathways, including Atoh1, Wnt, Hedgehog and Notch signaling. However, most new HCs are immature HCs. In this study we used RNA-Seq analysis to compare the differences between the transcriptomes of overexpression-induced new HCs and the native HCs, and to define the factors that might help to promote the maturation of new HCs. As expected, we found -induced new HCs had obvious HC characteristics as demonstrated by the expression of HC markers such as and Myosin VIIA (Myo7a). However, -induced new HCs had significantly lower expression of genes that are related to HC function such as Slc26a5 (), and . We found that genes related to HC cell differentiation and maturation (, , , , , , , , etc.) had significantly lower expression levels in new HCs compared to native HCs. In conclusion, we found a set of genes that might regulate the differentiation and maturation of new HCs, and these genes might serve as potential new therapeutic targets for functional HC regeneration and hearing recovery.

摘要

出生后的哺乳动物耳蜗毛细胞（HCs）可通过直接转分化或通过支持细胞经有丝分裂再生，再生途径包括Atoh1、Wnt、Hedgehog和Notch信号通路等多种。然而，大多数新生HCs是未成熟的HCs。在本研究中，我们使用RNA测序分析来比较过表达诱导产生的新生HCs与天然HCs转录组之间的差异，并确定可能有助于促进新生HCs成熟的因素。正如预期的那样，我们发现过表达诱导产生的新生HCs具有明显的HC特征，如通过HC标记物如和肌球蛋白VIIA（Myo7a）的表达所证明。然而，过表达诱导产生的新生HCs中与HC功能相关的基因如Slc26a5（）、和的表达显著较低。我们发现，与HC细胞分化和成熟相关的基因（、、、、、、、、等）在新生HCs中的表达水平与天然HCs相比显著较低。总之，我们发现了一组可能调节新生HCs分化和成熟的基因，这些基因可能成为功能性HC再生和听力恢复的潜在新治疗靶点。