使用单克隆抗体进行免疫层析测定，用于简单快速检测[病毒名称]及相关病毒。 （注：原文中“and related viruses”前缺少具体病毒名称，翻译时用[病毒名称]替代）
Immunochromatographic assay for simple and rapid detection of and related viruses using monoclonal antibodies.
作者信息
Kusano Nario, Hirashima Keita, Kuwahara Minoru, Narahara Kenji, Imamura Tadashi, Mimori Tomohiro, Nakahira Ken, Torii Kuniaki
机构信息
Fukuoka Agricultural Research Center, 587 Yoshiki, Chikushino, Fukuoka, 818-8549 Japan.
Mizuho Medy Co., Ltd., Tosu, Japan.
出版信息
J Gen Plant Pathol. 2007;73(1):66-71. doi: 10.1007/s10327-006-0316-6. Epub 2007 Feb 16.
Abstract
A simple and rapid immunochromatographic assay (ICA) to detect (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses.
摘要
利用抗猪德尔塔冠状病毒（SDV）单克隆抗体的胶体金偶联物，开发了一种简单快速的免疫层析检测法（ICA）来检测SDV。在测试的六种匀浆缓冲液中，0.1 M柠檬酸盐缓冲液（pH 7.0）在ICA检测中效果最佳。在ICA检测中，在酶联免疫吸附测定（ELISA）中广泛使用的匀浆缓冲液中添加0.1%巯基乙酸对反应有害，因为会导致胶体金出现不良凝聚。使用抗SDV单克隆抗体的ICA分别比双抗体夹心ELISA和使用抗SDV多克隆抗体的ICA灵敏8倍和16倍。该分析仅需15分钟即可完成。此外，使用抗SDV单克隆抗体的ICA还可以检测与SDV相关的病毒。
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