Kusano Nario, Hirashima Keita, Kuwahara Minoru, Narahara Kenji, Imamura Tadashi, Mimori Tomohiro, Nakahira Ken, Torii Kuniaki
Fukuoka Agricultural Research Center, 587 Yoshiki, Chikushino, Fukuoka, 818-8549 Japan.
Mizuho Medy Co., Ltd., Tosu, Japan.
J Gen Plant Pathol. 2007;73(1):66-71. doi: 10.1007/s10327-006-0316-6. Epub 2007 Feb 16.
A simple and rapid immunochromatographic assay (ICA) to detect (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses.
利用抗猪德尔塔冠状病毒（SDV）单克隆抗体的胶体金偶联物，开发了一种简单快速的免疫层析检测法（ICA）来检测SDV。在测试的六种匀浆缓冲液中，0.1 M柠檬酸盐缓冲液（pH 7.0）在ICA检测中效果最佳。在ICA检测中，在酶联免疫吸附测定（ELISA）中广泛使用的匀浆缓冲液中添加0.1%巯基乙酸对反应有害，因为会导致胶体金出现不良凝聚。使用抗SDV单克隆抗体的ICA分别比双抗体夹心ELISA和使用抗SDV多克隆抗体的ICA灵敏8倍和16倍。该分析仅需15分钟即可完成。此外，使用抗SDV单克隆抗体的ICA还可以检测与SDV相关的病毒。
