Wang Shaoyun, Rao Pingfan
Department of Food Science, Fuzhou University, Fuzhou, 350002 China.
Eur Food Res Technol. 2010;231(2):331-338. doi: 10.1007/s00217-010-1285-8. Epub 2010 Apr 28.
A trypsin-chymotrypsin inhibitor, designated , with both antifungal and antibacterial activity, and exhibiting a molecular mass of 18.0 kDa in SDS-polyacrylamide gel electrophoresis, was isolated from the large lima bean () legumes by a combination of extraction, ammonium sulfate precipitation, ion exchange chromatography on SP-Toyopearl and high performance liquid chromatography (HPLC) on Mono S. The isoelectric point was estimated to be 7.6 by isoelectric focusing The 15 N-terminal amino acid sequences were determined to be DFVIDNEGNPLENGG, demonstrating some resemblance to those other protease inhibitors and inhibitor precursors from leguminous plants. It exerted potent antifungal action toward , (.) , and It showed antiproliferative activity toward tumor cells including human liver hepatoma cells Bel-7402 and neuroblastoma cells SHSY5Y. However, it had no effect on bacteria and
从大莱豆（Phaseolus lunatus）豆科植物中，通过提取、硫酸铵沉淀、SP - Toyopearl离子交换色谱和Mono S高效液相色谱（HPLC）相结合的方法，分离出一种胰蛋白酶 - 糜蛋白酶抑制剂，命名为 ，它具有抗真菌和抗菌活性，在SDS - 聚丙烯酰胺凝胶电泳中显示分子量为18.0 kDa。通过等电聚焦估计其等电点为7.6。测定其15个N端氨基酸序列为DFVIDNEGNPLENGG，这表明它与豆科植物的其他蛋白酶抑制剂和抑制剂前体有一些相似之处。它对黑曲霉（Aspergillus niger）、寄生曲霉（Aspergillus parasiticus）、黄曲霉（Aspergillus flavus）具有强大的抗真菌作用。它对包括人肝癌细胞Bel - 7402和神经母细胞瘤细胞SHSY5Y在内的肿瘤细胞显示出抗增殖活性。然而，它对大肠杆菌（Escherichia coli）和 没有作用
