A leguminous trypsin-chymotrypsin inhibitor with antifungal activity from .

A trypsin-chymotrypsin inhibitor, designated , with both antifungal and antibacterial activity, and exhibiting a molecular mass of 18.0 kDa in SDS-polyacrylamide gel electrophoresis, was isolated from the large lima bean () legumes by a combination of extraction, ammonium sulfate precipitation, ion exchange chromatography on SP-Toyopearl and high performance liquid chromatography (HPLC) on Mono S. The isoelectric point was estimated to be 7.6 by isoelectric focusing The 15 N-terminal amino acid sequences were determined to be DFVIDNEGNPLENGG, demonstrating some resemblance to those other protease inhibitors and inhibitor precursors from leguminous plants. It exerted potent antifungal action toward , (.) , and It showed antiproliferative activity toward tumor cells including human liver hepatoma cells Bel-7402 and neuroblastoma cells SHSY5Y. However, it had no effect on bacteria and