双标记肽的固相合成：细胞可渗透的钙蛋白酶特异性底物的开发
Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates.
作者信息
Polster Brian M, Arze Rafael, Lyttle Matthew H, Nicholls David G, Hudson Derek
机构信息
2Buck Institute for Age Research, 8001 Redwood Blvd., Novato, CA 94945 USA.
1Biosearch Technologies Inc, 81 Digital Drive, Novato, CA 94949 USA.
出版信息
Int J Pept Res Ther. 2007;13(1):83-91. doi: 10.1007/s10989-006-9049-9. Epub 2006 Nov 30.
Abstract
A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.
摘要
本文报道了对用于蛋白酶钙蛋白酶的双标记荧光共振能量转移（FRET）底物的逐步评估。该研究产生了可穿透细胞的选择，对目标酶具有优化的特异性和有效性，并提高了对非特异性降解酶的稳定性。
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