Liu Chuanzhi, Liu Kai, Zhao Chunru, Gong Ping, Yu Yuanhua
School of Life Science and Technology, Changchun University of Science and Technology, Weixing Road 7989, Changchun, Jilin Province, 130022, PR China.
Toxicol Rep. 2020 Mar 6;7:460-467. doi: 10.1016/j.toxrep.2020.02.015. eCollection 2020.
is a research topic that can degrade steroid hormones into water and carbon dioxide through a series of enzymes in the body. Short-chain dehydrogenase (SDR) are a class of NAD (P) H-dependent oxidoreductases in C. . Its main function is catalyzing the redox of the hydroxyl/ketone group of the hormone. In this paper, a SDR gene() is cloned from C. ATCC11996 and expressed. The polyclonal antibody was prepared and the gene knocked out by homologous recombination. Wild type and mutant C. induced by testosterone, estradiol, estrone and estriol. The growth curves of the bacteria were measured by spectrophotometer. ELISA established the expression of SDRx protein, and high-performance liquid chromatography(HPLC) detected the contents of various hormones. The results show that the growth of wild type was faster than mutant type induced by testosterone. The concentration of SDRx is 0.318 mg/ml under testosterone induction. It has a great change in steroid hormones residue in culture medium measured by HPLC: Testosterone residue in the mutant type group was 42.4 % more than the wild type in culture medium. The same thing happens with induced by estrone. In summary, this gene involved in the degradation of testosterone and estradiol, and effects the growth of .
是一个研究课题，其可以通过体内一系列酶将类固醇激素降解为水和二氧化碳。短链脱氢酶（SDR）是梭菌属中的一类NAD（P）H依赖性氧化还原酶。其主要功能是催化激素羟基/酮基的氧化还原反应。本文从梭菌属ATCC11996中克隆并表达了一个SDR基因（）。制备了多克隆抗体，并通过同源重组敲除了该基因。用睾酮、雌二醇、雌酮和雌三醇诱导野生型和突变型梭菌。用分光光度计测量细菌的生长曲线。酶联免疫吸附测定（ELISA）确定SDRx蛋白的表达，高效液相色谱法（HPLC）检测各种激素的含量。结果表明，睾酮诱导下野生型的生长速度比突变型快。在睾酮诱导下，SDRx的浓度为0.318mg/ml。通过HPLC测定，其在培养基中类固醇激素残留量有很大变化：突变型组培养基中睾酮残留量比野生型多42.4%。雌酮诱导时也出现同样情况。综上所述，该基因参与睾酮和雌二醇的降解，并影响梭菌的生长。
