Uchiyama T, Uchida T
Department of Virology, School of Medicine, University of Tokushima.
Microbiol Immunol. 1988;32(11):1163-6. doi: 10.1111/j.1348-0421.1988.tb01479.x.
Japanese isolates of spotted fever group rickettsiae were observed under a transmission electron microscope. In Vero cells persistently infected with Japanese isolates, small numbers of intracytoplasmic rickettsiae were seen. On the other hand, moderate numbers of rickettsiae were found in the cytoplasm of productively infected BHK cells. The electron-lucent, halo-like zone was found to surround organisms in the cytoplasm of their host cells, which is a prominent characteristic of spotted fever group rickettsiae. Fine structural features of the cell wall revealed thin outer and thick inner leaflets like those observed in other spotted fever group rickettsiae.
利用透射电子显微镜对日本斑点热群立克次体分离株进行了观察。在持续感染日本分离株的Vero细胞中，可见少量胞质内立克次体。另一方面，在高效感染的BHK细胞的细胞质中发现了中等数量的立克次体。在宿主细胞的细胞质中发现，立克次体周围存在电子透明的晕样区域，这是斑点热群立克次体的一个显著特征。细胞壁的精细结构特征显示，其外叶薄、内叶厚，与其他斑点热群立克次体观察到的情况一样。
