Beamont A H, Miguel A, Goos C M, Vermeesch-Markslag A M, Hermans A, Vermorken A J
Research Unit for Cellular Differentiation and Transformation, University of Nijmegen, The Netherlands.
Mol Biol Rep. 1988;13(2):73-8. doi: 10.1007/BF00539053.
Saliva was investigated for its suitability as a biopsy tissue for the determination of glucose-6-phosphate dehydrogenase deficiency. It appears that there is a significant difference between the activity of the enzyme in patients and controls. However, some controls have very low values making discrimination between patients and controls using a qualitative method impossible. Glucose-6-phosphate dehydrogenase deficiency is a relevant clinical problem in many rural areas in developing countries. Existing methods for determination of the deficiency in blood and hair follicles do not meet the criteria necessary for their large scale introduction in the areas of the world that are concerned by the problem. The present study shows that saliva is not a suitable alternative. Between the three biopsy tissues compared: blood, hair follicles and saliva, hair follicles remain most attractive since their isolation hardly involves the risk of infection. A simplified method for the detection of glucose-6-phosphate dehydrogenase activity in hair follicles that would allow health service workers in the field to determine the carrier status of pregnant women might form the basis for a future kernicterus prevention programme.
对唾液作为用于测定葡萄糖-6-磷酸脱氢酶缺乏症的活检组织的适用性进行了研究。结果显示，患者和对照组中该酶的活性存在显著差异。然而，一些对照组的值非常低，使得使用定性方法区分患者和对照组变得不可能。葡萄糖-6-磷酸脱氢酶缺乏症在发展中国家的许多农村地区是一个相关的临床问题。现有的测定血液和毛囊中该缺乏症的方法不符合在世界上受该问题影响的地区大规模推广所需的标准。本研究表明唾液不是合适的替代物。在比较的三种活检组织（血液、毛囊和唾液）中，毛囊仍然最具吸引力，因为分离毛囊几乎不涉及感染风险。一种用于检测毛囊中葡萄糖-6-磷酸脱氢酶活性的简化方法，该方法将使现场的卫生服务人员能够确定孕妇的携带者状态，这可能构成未来预防核黄疸计划的基础。
