Deepankumar Kanagavel, Nadarajan Saravanan Prabhu, Bae Dong-Ho, Baek Kwang-Hyun, Choi Kwon-Young, Yun Hyungdon
1School of Biotechnology, Yeungnam University, Gyeongsan, 712-749 Korea.
2Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 Korea.
Biotechnol Bioprocess Eng. 2015;20(1):67-72. doi: 10.1007/s12257-014-0456-z. Epub 2015 Mar 18.
Genetically encoded fluorescent proteins are extensively utilized for labeling and imaging proteins, organelles, cell tissues, and whole organisms. In this study, we explored the feasibility of mRFP1 and its variants for measuring intracellular temperature. A linear relationship was observed between the temperature and fluorescence intensity of mRFP1 and its variants. Temperature sensitivities of expressing mRFP1, mRFP-P63A and mRFP-P63A[(4)-FP] were -1.27%, -1.26% and -0.77%/°C, respectively. Finally, we demonstrated the potentiality of mRFP1 and its variants as an temperature sensor.
基因编码荧光蛋白被广泛用于标记和成像蛋白质、细胞器、细胞组织及整个生物体。在本研究中，我们探究了mRFP1及其变体用于测量细胞内温度的可行性。观察到mRFP1及其变体的温度与荧光强度之间存在线性关系。表达mRFP1、mRFP-P63A和mRFP-P63A[(4)-FP]的温度敏感性分别为-1.27%、-1.26%和-0.77%/℃。最后，我们证明了mRFP1及其变体作为温度传感器的潜力。
