Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yeonjudanji-ro 30, Chungbuk 28116, Korea.
Department of Public Health, College of Natural Science, Keimyung University, Daegu, 42601, Korea.
Int J Med Sci. 2020 Mar 12;17(6):815-823. doi: 10.7150/ijms.40697. eCollection 2020.
Importin-11 (Ipo11) is a novel member of the human importin family of transport receptors (karyopherins), which are known to mediate the nucleocytoplasmic transport of protein and RNA cargos. Despite its role in the transport of protein, we found that knockout of Ipo11 nuclear import factor affects normal embryonic development and govern embryo-lethal phenotypes in mice. In this study, we for the first time produced a mouse line containing null mutation in gene utilized by gene trapping. The embryos showed an embryonic lethal phenotype. The embryos showed a reduced size at embryonic day 10.5 (E10.5) when compared with or embryos and died by E11.5. Whereas mice were healthy and fertile, and there was no detectable changes in embryonic lethality and phenotype when reviewed. In the X-gal staining with the or embryos, strong X-gal staining positivity was detected systematically in the whole mount embryos at E10.5, although almost no X-gal positivity was detected at E9.5, indicating that the embryos die soon after the process of Ipo11 expression started. These results indicate that Ipo11 is essential for the normal embryonic development in mice.
Importin-11 (Ipo11) 是人类 importin 家族转运受体（核输入蛋白）的一个新成员，已知其介导蛋白质和 RNA 货物的核质转运。尽管 Ipo11 是一种核输入蛋白，但我们发现其敲除会影响正常胚胎发育，并导致小鼠胚胎致死表型。在这项研究中，我们首次利用基因捕获产生了一种缺失突变的小鼠品系。该突变基因编码 Importin-11（Ipo11）。Ipo11 基因敲除的胚胎表现出胚胎致死表型。与野生型或杂合子胚胎相比，Ipo11 基因敲除的胚胎在 E10.5 时体积减小，并在 E11.5 时死亡。而 Ipo11 杂合子小鼠是健康且有生育能力的，且在胚胎致死率和表型方面没有明显变化。在 X-gal 染色方面，Ipo11 基因敲除的胚胎在 E10.5 时在整个胚胎中都呈现强烈的 X-gal 阳性染色，但在 E9.5 时几乎没有 X-gal 阳性染色，这表明在 Ipo11 表达开始后不久，胚胎就死亡了。这些结果表明，Ipo11 对小鼠正常胚胎发育是必需的。
