Miller L H, Hudson D, Haynes J D
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Mol Biochem Parasitol. 1988 Dec;31(3):217-22. doi: 10.1016/0166-6851(88)90151-x.
Plasmodium knowlesi, a malaria of Old World monkeys, invades all Duffy blood group positive human erythrocytes and various New World monkey erythrocytes except Cebus apella. We had previously identified a 135 kDa parasite protein in supernatants of P. knowlesi cultures that bound to Duffy positive but not to Duffy negative human erythrocytes [Haynes et al., J. Exp. Med. 167, 1873-1881 (1988)]. We now use New World monkey erythrocytes as a reagent to identify P. knowlesi proteins in culture supernatants that will bind to all New World monkey erythrocytes susceptible to invasion but not to C. apella erythrocytes, which are refractory to invasion. The 135 kDa protein binds to all New World monkey erythrocytes, including C. appella. Another protein of 155 kDa binds to all New World monkey erythrocytes except C. apella. The 155 kDa protein binds to Old World monkey erythrocytes, the natural host of P. knowlesi; it does not bind to human Duffy positive erythrocytes. This and the previous study are the beginning of the identification of parasite proteins of P. knowlesi that bind to erythrocytes in a receptor specific manner.
诺氏疟原虫是一种旧大陆猴疟疾，可侵入所有达菲血型阳性的人类红细胞以及除僧帽猴外的各种新大陆猴红细胞。我们之前在诺氏疟原虫培养上清液中鉴定出一种135 kDa的寄生虫蛋白，该蛋白可与达菲阳性而非达菲阴性的人类红细胞结合[海恩斯等人，《实验医学杂志》167, 1873 - 1881 (1988)]。我们现在使用新大陆猴红细胞作为试剂，来鉴定培养上清液中能与所有易被侵入的新大陆猴红细胞结合，但不与不易被侵入的僧帽猴红细胞结合的诺氏疟原虫蛋白。135 kDa的蛋白能与所有新大陆猴红细胞结合，包括僧帽猴红细胞。另一种155 kDa的蛋白能与除僧帽猴外的所有新大陆猴红细胞结合。155 kDa的蛋白能与诺氏疟原虫的天然宿主旧大陆猴红细胞结合；它不与人类达菲阳性红细胞结合。这项研究以及之前的研究是鉴定诺氏疟原虫以受体特异性方式与红细胞结合的寄生虫蛋白的开端。
