Central Laboratory, Danyang People's Hospital of Jiangsu Province, Danyang, Jiangsu, China.
Med Oncol. 2020 Mar 27;37(5):34. doi: 10.1007/s12032-020-01362-0.
Gastric cancer (GC) is one of the most common malignant tumors in the world, and it is also the third largest cause of cancer-related death in the world. As far as we know, no biomarker has been widely accepted for early diagnosis and prognosis prediction of gastric cancer. The purpose of this study is to find potential biomarkers to predict the prognosis of GC. The gene expression profiles of GSE2685 were downloaded from GEO database. Morpheus was used to calculate the differentially expressed genes (DEGs) between primary advanced gastric cancer tissues and noncancerous gastric tissues. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and protein-protein interaction (PPI) network of DEGs was constructed. Kaplan-Meier Plotter was used to determine the overall survival (OS) outcomes of UC5AC, MUC1, KRT7, GAPDH, CD44, and GEPIA was used to determine the Pearson correlation analysis. In total, 710 DEGs were identified in GC, including 396 upregulated genes and 314 downregulated genes. GO enrichment revealed that they were mainly enriched in binding, catalytic activity, cellular process and cell. KEGG pathway revealed that they were mainly enriched in metabolic pathways, pathways in cancer and PI3K-Akt signaling pathway. MUC5AC, MUC1, KRT7, GAPDH, CD44 were identified from the PPI network. MUC5AC, MUC1, KRT7, GAPDH, CD44 were demonstrated to have prognostic value for patients with GC. MUC5AC, MUC1 exhibited low expression levels in GC tissues, KRT7, GAPDH, CD44 presented high expression levels in GC tissues. In particular, KRT7 is hardly expressed in normal gastric tissues. MUC5AC and MUC1 were negatively correlated with GAPDH, CD44, respectively; and GAPDH was positively correlated with CD44 and KRT7, respectively. Moreover. MUC5AC, MUC1, KRT7, GAPDH, and CD44 are not only related to GC but also to apoptosis pathway. Results from the present study suggested that MUC5AC, MUC1, KRT7, GAPDH, CD44 may represent novel prognostic biomarkers for GC.
胃癌（GC）是世界上最常见的恶性肿瘤之一，也是世界上癌症相关死亡的第三大原因。据我们所知，目前还没有一种生物标志物被广泛接受用于胃癌的早期诊断和预后预测。本研究的目的是寻找潜在的生物标志物来预测 GC 的预后。从 GEO 数据库中下载了 GSE2685 的基因表达谱。Morpheus 用于计算原发性晚期胃癌组织与非癌性胃组织之间的差异表达基因（DEGs）。进行了基因本体论（GO）和京都基因与基因组百科全书通路（KEGG）富集分析，并构建了 DEGs 的蛋白质-蛋白质相互作用（PPI）网络。Kaplan-Meier Plotter 用于确定 UC5AC、MUC1、KRT7、GAPDH、CD44 的总生存期（OS）结果，GEPIA 用于确定 Pearson 相关分析。总共在 GC 中鉴定出 710 个 DEGs，包括 396 个上调基因和 314 个下调基因。GO 富集显示它们主要富集在结合、催化活性、细胞过程和细胞中。KEGG 通路显示它们主要富集在代谢途径、癌症途径和 PI3K-Akt 信号通路中。从 PPI 网络中鉴定出 MUC5AC、MUC1、KRT7、GAPDH 和 CD44。MUC5AC、MUC1、KRT7、GAPDH 和 CD44 被证明对 GC 患者具有预后价值。MUC5AC、MUC1 在 GC 组织中表达水平较低，KRT7、GAPDH、CD44 在 GC 组织中表达水平较高。特别是，KRT7 在正常胃组织中几乎不表达。MUC5AC 与 GAPDH、CD44 分别呈负相关；而 GAPDH 与 CD44 和 KRT7 分别呈正相关。此外，MUC5AC、MUC1、KRT7、GAPDH 和 CD44 不仅与 GC 有关，还与细胞凋亡途径有关。本研究结果表明，MUC5AC、MUC1、KRT7、GAPDH、CD44 可能代表 GC 的新型预后生物标志物。
