Department of Cardiology Faculty of Medicine, Nigde Omer Halisdemir University, Nigde, Turkey.
Department of Medical Biology, Faculty of Medicine, Ömer Halisdemir University, Nigde, Turkey.
Methods Mol Biol. 2020;2138:373-379. doi: 10.1007/978-1-0716-0471-7_27.
Apolipoproteins have important structural and functional roles in several lipoprotein particles. Apolipoproteins regulate lipid metabolism, adipose tissue, and energy production and serve major regulatory roles in both pre- and pro-atherosclerotic mechanisms. They are also involved in protective mechanisms against atherosclerotic plaques. Therefore, accurate quantification of apolipoproteins may serve as a crucial biomarker for cardiovascular diseases. However, most apolipoproteins cannot be detected using standard clinical immunoassays, and multiplexing is not available for some species of apolipoproteins. Herein, we describe a highly robust and quantitative method using liquid chromatography coupled to tandem mass spectrometry to quantify apolipoproteins in plasma. This methodology may add clinical value for profiling cardiovascular risk in vulnerable individuals and enable monitoring of apolipoprotein levels in plasma following intervention strategies.
载脂蛋白在几种脂蛋白颗粒中具有重要的结构和功能作用。载脂蛋白调节脂质代谢、脂肪组织和能量产生，并在动脉粥样硬化前和前体机制中发挥主要调节作用。它们还参与对抗动脉粥样硬化斑块的保护机制。因此，载脂蛋白的准确定量可以作为心血管疾病的关键生物标志物。然而，大多数载脂蛋白不能使用标准的临床免疫测定法检测，并且某些载脂蛋白的多重检测不可用。在此，我们描述了一种使用液相色谱串联质谱联用技术定量检测血浆中载脂蛋白的高度稳健和定量方法。这种方法学可能为易受影响的个体进行心血管风险分析增加临床价值，并能够在干预策略后监测血浆中载脂蛋白水平。
