Prasetyaningtyas Wahono Esthi, Karja Ni Wayan Kurniani, Agungpriyono Srihadi, Fahrudin Mokhamad
Graduate Program in Animal Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Bogor, Indonesia.
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Bogor, Indonesia.
Anim Sci J. 2020 Jan-Dec;91(1):e13332. doi: 10.1111/asj.13332.
The crude testicular cells (CTCs) contain many cell types, such as Sertoli cells, leydig cells, spermatogonial stem cells (SSCs), spermatocytes, and other somatic testicular cells, that secrete various growth factors needed in spermatogenesis. The objective of this study was to characterize development of 5-day-old mice testicular cells cultured. Crude testicular cells prepared from the testes of 5-day-old male mice were cultured in Dulbecco's Modified Eagle Medium and incubated at 37°C in a 5% CO atmosphere for 6 days. The results demonstrated that the testicular cells developed rapidly with a population doubling time (PDT) of 0.63 days and more than 90% of cells were viable after being cultured for 3 days. The number of Sertoli-like cells increased significantly over days 1, 3, and 6 to 22.1%, 34.6%, and 50.1%, respectively. A significant increase was also observed in fibroblast-like cells (15.5% on day 1 to 28.8% on day 3 and to 26.6% on day 6). In contrast, the number of spermatogonia-like cells decreased significantly (54.3%, 30.4%, and 18.7%, on days 1, 3, and 6, respectively). These data indicated that the developmental pattern of the testicular cell in this study might be affected by the niche provided by the cultured testicular cells.
粗制睾丸细胞（CTCs）包含多种细胞类型，如支持细胞、间质细胞、精原干细胞（SSCs）、精母细胞以及其他睾丸体细胞，这些细胞分泌精子发生过程中所需的各种生长因子。本研究的目的是对培养的5日龄小鼠睾丸细胞的发育进行表征。从5日龄雄性小鼠的睾丸中制备的粗制睾丸细胞在杜尔贝科改良伊格尔培养基中培养，并在37°C、5% CO₂气氛中孵育6天。结果表明，睾丸细胞发育迅速，群体倍增时间（PDT）为0.63天，培养3天后超过90%的细胞存活。支持样细胞的数量在第1天、第3天和第6天显著增加，分别达到22.1%、34.6%和50.1%。成纤维样细胞也有显著增加（从第1天的15.5%增加到第3天的28.8%，再到第6天的26.6%）。相比之下，精原样细胞的数量显著减少（分别在第1天、第3天和第6天为54.3%、30.4%和18.7%）。这些数据表明，本研究中睾丸细胞的发育模式可能受到培养的睾丸细胞所提供的微环境的影响。
