School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2020 Aug 1;320:126647. doi: 10.1016/j.foodchem.2020.126647. Epub 2020 Mar 19.
In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH)SO) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods. Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH)SO after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH)SO, respectively. OPS-50 had lower weight-average molecular weight (3.65 × 10 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity. Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.
在这项研究中，首次采用三相分配（TPP）结合梯度硫酸铵（（NH 4 ） 2 SO 4 ）沉淀（GNP）法从新鲜的秋葵荚中提取和分离生物活性多糖（OPSs）。结果表明，通过 GNP 在 TPP 后达到 50%（NH 4 ） 2 SO 4 饱和度获得的 OPS-50 具有较高的沉淀产率、碳水化合物（86.82%）和糖醛酸（22.34%）含量，与通过 GNP 在饱和度为 30%和 40%（NH 4 ） 2 SO 4 获得的 OPS-30 和 OPS-40 相比，具有相同的单糖组成和不同的摩尔比。与 OPS-30、OPS-40 和 TPP 平行化得到的 OPS 相比，OPS-50 的重均分子量（3.65×10 4 g/mol）较低，链更具柔韧性，表面结构相对较松散，表现出较低的表观粘度和动态粘弹性。此外，OPS-50 在体外具有更强的 DPPH 自由基清除能力、抗氧化能力以及对α-淀粉酶和α-葡萄糖苷酶的抑制活性。
