Department of Environmental Technology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jalisco, Mexico.
Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Bioresour Technol. 2020 Jul;307:123160. doi: 10.1016/j.biortech.2020.123160. Epub 2020 Mar 7.
This study evaluated a novel three-stage process devoted to the cascade production of lactate, biohydrogen and methane from tequila vinasse (TV), with emphasis on attaining a high and stable biohydrogen production rate (HPR) by utilizing lactate as biohydrogen precursor. In the first stage, tailored operating conditions applied to a sequencing batch reactor were effective in sustaining a lactate concentration of 12.4 g/L, corresponding to 89% of the total organic acids produced. In the second stage, the stimulation of lactate-centered dark fermentation which entails the decoupling of biohydrogen production from carbohydrates utilization was an effective approach enabling stable biohydrogen production, having HPR fluctuations less than 10% with a maximum HPR of 12.3 L/L-d and a biohydrogen yield of 3.1 L/L. Finally, 1.6 L CH/L-d and 6.5 L CH/L were obtained when feeding the biohydrogen fermentation effluent to a third methanogenic stage, yielding a global energy recovery of 267.5 kJ/L.
本研究评估了一种新颖的三阶段工艺，用于从龙舌兰酒废液（TV）连续生产乳酸、生物氢和甲烷，重点是利用乳酸作为生物氢前体来实现高且稳定的生物氢生产速率（HPR）。在第一阶段，适用于序批式反应器的定制操作条件有效地维持了 12.4 g/L 的乳酸浓度，相当于产生的总有机酸的 89%。在第二阶段，刺激以乳酸为中心的黑暗发酵，使生物氢生产与碳水化合物利用解耦，是一种有效的方法，可以实现稳定的生物氢生产，HPR 波动小于 10%，最大 HPR 为 12.3 L/L-d，生物氢产率为 3.1 L/L。最后，当将生物氢发酵的流出物进料到第三甲烷化阶段时，获得了 1.6 L CH/L-d 和 6.5 L CH/L，总能量回收率为 267.5 kJ/L。
