School of Civil Engineering and Construction and Environment, Xihua University, Chengdu 610039, P.R. China.
School of Food and Biotechnology, Xihua University, Chengdu 610039, P.R. China.
Can J Microbiol. 2020 Aug;66(8):475-489. doi: 10.1139/cjm-2019-0632. Epub 2020 Mar 30.
The aim of this study was to investigate the effects of a volcanic rock porous carrier (VRPC) on sludge reduction, pollutant removal, and microbial community structure in an anaerobic side-stream reactor (ASSR). Three lab-scale membrane bioreactors (MBRs), including an anoxic-oxic MBR, which served as the control (C-MBR), an ASSR-coupled MBR (A-MBR), and an A-MBR filled with VRPC (FA-MBR) were stably and simultaneously operated for 120 days. The effect of the three reactors on the removal of chemical oxygen demand (COD) was almost negligible (all greater than 95%), but the average removal efficiency of ammonium nitrogen, total nitrogen, and total phosphorus was significantly improved by the insertion of an ASSR, especially when the ASSR was filled with VRPC. Finally, A-MBR and FA-MBR achieved 16.2% and 26.4% sludge reduction rates, with observed sludge yields of 0.124 and 0.109 g mixed liquid suspended solids/g COD, respectively. Illumina MiSeq sequencing revealed that microbial diversity and richness were highest in the VRPC, indicating that a large number of microorganisms formed on the carrier surface in the form of a biofilm. Abundant denitrifying bacteria (, unclassified, and ) were immobilized on the carrier biofilm, which contributed to increased nitrogen removal. The addition of a VRPC to the ASSR successfully immobilized abundant hydrolytic, fermentative, and slow-growing microorganisms, which all contributed to reductions in sludge yield.
本研究旨在考察火山岩多孔载体（VRPC）对厌氧侧流反应器（ASSR）中污泥减量、污染物去除和微生物群落结构的影响。三个实验室规模的膜生物反应器（MBR），包括缺氧-好氧 MBR 作为对照（C-MBR）、耦合 ASSR 的 MBR（A-MBR）和填充 VRPC 的 A-MBR（FA-MBR），稳定且同时运行了 120 天。三个反应器对化学需氧量（COD）去除效果几乎可以忽略不计（均大于 95%），但插入 ASSR 可显著提高氨氮、总氮和总磷的平均去除效率，尤其是当 ASSR 填充 VRPC 时。最终，A-MBR 和 FA-MBR 的污泥减率分别达到 16.2%和 26.4%，观察到的污泥产率分别为 0.124 和 0.109 g 混合液悬浮固体/g COD。Illumina MiSeq 测序表明，VRPC 中的微生物多样性和丰富度最高，表明大量微生物以生物膜的形式在载体表面形成。丰富的反硝化菌（，未分类和）被固定在载体生物膜上，这有助于提高氮去除率。在 ASSR 中添加 VRPC 成功固定了大量的水解、发酵和缓慢生长的微生物，这都有助于降低污泥产率。
